• Cart
    • Quote
    • Enquiry
    • Cart
    • Quote
    • Enquiry
Novogene Europe
  • Novogene Europe
  • Genomics
    • Human Whole Genome Sequencing
    • Whole Exome Sequencing
    • Plant and Animal Whole Genome Sequencing
    • Plant and Animal De novo Sequencing
    • Microbial Whole Genome Sequencing
    • Microbial De novo Sequencing
    • Whole Plasmid SequencingOrder Online!
    Proteomics
    • Olink ProteomicsNew!
    Epigenomics
    • DNA Methylation SequencingUpdated!
    • Chromatin Immunoprecipitation Sequencing (ChIP-seq)
    • RNA Immunoprecipitation Sequencing (RIP-seq)
    Metabolomics
    • Untargeted MetabolomicsComing Soon!
    Transcriptomics
    • mRNA Sequencing
    • Small RNA Sequencing (sRNA‑seq)
    • Circular RNA Sequencing (circRNA-seq)
    • Total RNA Sequencing
    • Whole Transcriptome Sequencing
    • Full-Length Transcriptome Sequencing
    • Prokaryotic RNA Sequencing
    • Metatranscriptome Sequencing
    Metagenomics
    • Amplicon SequencingOrder Online!
    • Shotgun Metagenomics Sequencing
    Single Cell & Spatial Omics
    • 10X Single Cell Gene Expression
    • 10X Single Cell Immune Profiling
    • 10X Visium HD Spatial Gene Expression
    Premade Library
    • Sequencing Only on Illumina® Sequencer
    • Sequencing Only on Element SequencerNew!
    • Sequencing Only on PacBio Sequencer
    Translational Research
    • Accredited & Validated Clinical Research Sequencing
  • PromotionsPromotions
    • Platforms
    • Automated Delivery Platform (Falcon)
    • Bioinformatics Analysis Tool (NovoMagic)
    • Customer Service System (CSS)
    • Customer Support
    • Webinars
    • Case Study
    • Blog
    • Brochure
    • Cancer Research
    • Immuno-oncology
    • Agrigenomics
    • Environment
    • Food Science
    • Human Microbiome
    • Plant and Animal Microbiome
    • Drug Discovery and Development
    • Rare and Complex Diseases
    • About Us
    • News & Events
    • Careers
    • Our Locations
  • Contact UsContact Us
    • Amplicon Sequencing
    • Whole Plasmid Sequencing

ServicesServices menu

ResourcesResources menu

SupportSupport menu

CompanyCompany menu

Services
Human Whole Genome SequencingWhole Exome SequencingPlant and Animal Whole Genome SequencingPlant and Animal De novo SequencingDNA Methylation SequencingmRNA SequencingFull-Length Transcriptome SequencingWhole Transcriptome SequencingMetatranscriptome SequencingShotgun Metagenomics SequencingAmplicon SequencingWhole Plasmid Sequencing10X Single Cell Gene Expression10X Single Cell Immune Profiling10X Visium HD Spatial Gene ExpressionOlink ProteomicsUntargeted MetabolomicsAccredited & Validated Clinical Research Sequencing
Resources
WebinarsCase StudyBlogBrochure
Support
PlatformBioinformatics Analysis Tool (NovoMagic)Customer Service System (CSS)Customer Support
Company
About UsNews & EventsLocationContact Us
LinkedInLinkedIn hoverYouTubeYouTube hoverInstagramInstagram hoverXX hover
Copyright © 2026 Novogene Inc. All rights reserved.For Research Use Only. Not for Clinical Diagnostic Use.
    • Cart
    • Quote
    • Enquiry
    • Cart
    • Quote
    • Enquiry
Novogene Europe
  • Novogene Europe
  • Genomics
    • Human Whole Genome Sequencing
    • Whole Exome Sequencing
    • Plant and Animal Whole Genome Sequencing
    • Plant and Animal De novo Sequencing
    • Microbial Whole Genome Sequencing
    • Microbial De novo Sequencing
    • Whole Plasmid SequencingOrder Online!
    Proteomics
    • Olink ProteomicsNew!
    Epigenomics
    • DNA Methylation SequencingUpdated!
    • Chromatin Immunoprecipitation Sequencing (ChIP-seq)
    • RNA Immunoprecipitation Sequencing (RIP-seq)
    Metabolomics
    • Untargeted MetabolomicsComing Soon!
    Transcriptomics
    • mRNA Sequencing
    • Small RNA Sequencing (sRNA‑seq)
    • Circular RNA Sequencing (circRNA-seq)
    • Total RNA Sequencing
    • Whole Transcriptome Sequencing
    • Full-Length Transcriptome Sequencing
    • Prokaryotic RNA Sequencing
    • Metatranscriptome Sequencing
    Metagenomics
    • Amplicon SequencingOrder Online!
    • Shotgun Metagenomics Sequencing
    Single Cell & Spatial Omics
    • 10X Single Cell Gene Expression
    • 10X Single Cell Immune Profiling
    • 10X Visium HD Spatial Gene Expression
    Premade Library
    • Sequencing Only on Illumina® Sequencer
    • Sequencing Only on Element SequencerNew!
    • Sequencing Only on PacBio Sequencer
    Translational Research
    • Accredited & Validated Clinical Research Sequencing
  • PromotionsPromotions
    • Platforms
    • Automated Delivery Platform (Falcon)
    • Bioinformatics Analysis Tool (NovoMagic)
    • Customer Service System (CSS)
    • Customer Support
    • Webinars
    • Case Study
    • Blog
    • Brochure
    • Cancer Research
    • Immuno-oncology
    • Agrigenomics
    • Environment
    • Food Science
    • Human Microbiome
    • Plant and Animal Microbiome
    • Drug Discovery and Development
    • Rare and Complex Diseases
    • About Us
    • News & Events
    • Careers
    • Our Locations
  • Contact UsContact Us
    • Amplicon Sequencing
    • Whole Plasmid Sequencing

ServicesServices menu

ResourcesResources menu

SupportSupport menu

CompanyCompany menu

Services
Human Whole Genome SequencingWhole Exome SequencingPlant and Animal Whole Genome SequencingPlant and Animal De novo SequencingDNA Methylation SequencingmRNA SequencingFull-Length Transcriptome SequencingWhole Transcriptome SequencingMetatranscriptome SequencingShotgun Metagenomics SequencingAmplicon SequencingWhole Plasmid Sequencing10X Single Cell Gene Expression10X Single Cell Immune Profiling10X Visium HD Spatial Gene ExpressionOlink ProteomicsUntargeted MetabolomicsAccredited & Validated Clinical Research Sequencing
Resources
WebinarsCase StudyBlogBrochure
Support
PlatformBioinformatics Analysis Tool (NovoMagic)Customer Service System (CSS)Customer Support
Company
About UsNews & EventsLocationContact Us
LinkedInLinkedIn hoverYouTubeYouTube hoverInstagramInstagram hoverXX hover
Copyright © 2026 Novogene Inc. All rights reserved.For Research Use Only. Not for Clinical Diagnostic Use.
banner laptop bg
banner mobile bg

Circular RNA Sequencing (circRNA-seq)

Profiling circular RNAs to explore back-splice junctions, non-coding RNA regulation and expression changes across biological conditions.
OverviewOverview
BenefitsBenefits
ApplicationsApplications
SpecificationsSpecifications
ResourcesResources

Circular RNA sequencing, also known as circRNA-seq, enables the identification and quantification of circular RNAs generated through back-splicing events. By enriching and sequencing circular transcripts, circRNA-seq helps researchers detect back-splice junctions and compare circRNA expression across samples or experimental conditions.


Novogene Europe supports circRNA-seq projects using qualified total RNA inputs, library preparation workflows designed for circular RNA enrichment, high-throughput sequencing and bioinformatics analysis. The service supports circRNA identification, expression quantification, differential expression analysis, functional enrichment and target gene prediction.

Service Highlights

Circular Transcript Profiling
Circular Transcript Profiling

Profile circular RNAs across samples and conditions to explore non-coding RNA regulation, expression changes and biological responses across different study groups.

Circular Transcript Profiling
Circular Transcript Profiling

Profile circular RNAs across samples and conditions to explore non-coding RNA regulation, expression changes and biological responses across different study groups.

Circular RNA Enrichment Workflow
Circular RNA Enrichment Workflow

Library preparation workflows are designed to enrich circular RNA signals from qualified RNA inputs, supporting downstream sequencing and circRNA-focused analysis.

Circular RNA Enrichment Workflow
Circular RNA Enrichment Workflow

Library preparation workflows are designed to enrich circular RNA signals from qualified RNA inputs, supporting downstream sequencing and circRNA-focused analysis.

Back-splice Junction Identification
Back-splice Junction Identification

Bioinformatics analysis supports detection of back-splice junctions, helping identify circular transcripts and distinguish circRNA signals from linear RNA.

Back-splice Junction Identification
Back-splice Junction Identification

Bioinformatics analysis supports detection of back-splice junctions, helping identify circular transcripts and distinguish circRNA signals from linear RNA.

Regulatory and Functional Insight
Regulatory and Functional Insight

Analyse differential circRNA expression, predicted target interactions and functional enrichment to support biological interpretation of circRNA-associated regulatory changes.

Regulatory and Functional Insight
Regulatory and Functional Insight

Analyse differential circRNA expression, predicted target interactions and functional enrichment to support biological interpretation of circRNA-associated regulatory changes.

Service Highlights

Circular Transcript Profiling
Circular Transcript Profiling

Profile circular RNAs across samples and conditions to explore non-coding RNA regulation, expression changes and biological responses across different study groups.

Circular Transcript Profiling
Circular Transcript Profiling

Profile circular RNAs across samples and conditions to explore non-coding RNA regulation, expression changes and biological responses across different study groups.

Circular RNA Enrichment Workflow
Circular RNA Enrichment Workflow

Library preparation workflows are designed to enrich circular RNA signals from qualified RNA inputs, supporting downstream sequencing and circRNA-focused analysis.

Circular RNA Enrichment Workflow
Circular RNA Enrichment Workflow

Library preparation workflows are designed to enrich circular RNA signals from qualified RNA inputs, supporting downstream sequencing and circRNA-focused analysis.

Back-splice Junction Identification
Back-splice Junction Identification

Bioinformatics analysis supports detection of back-splice junctions, helping identify circular transcripts and distinguish circRNA signals from linear RNA.

Back-splice Junction Identification
Back-splice Junction Identification

Bioinformatics analysis supports detection of back-splice junctions, helping identify circular transcripts and distinguish circRNA signals from linear RNA.

Regulatory and Functional Insight
Regulatory and Functional Insight

Analyse differential circRNA expression, predicted target interactions and functional enrichment to support biological interpretation of circRNA-associated regulatory changes.

Regulatory and Functional Insight
Regulatory and Functional Insight

Analyse differential circRNA expression, predicted target interactions and functional enrichment to support biological interpretation of circRNA-associated regulatory changes.

Applications

Regulatory Response Analysis

circRNA-seq helps explore circular RNA changes linked to development, disease, treatment response or cellular adaptation across different biological conditions.

Regulatory Response Analysis

circRNA-seq helps explore circular RNA changes linked to development, disease, treatment response or cellular adaptation across different biological conditions.

Non-coding RNA Regulation

Circular RNA profiles can support studies of non-coding RNA regulation, including circRNA–miRNA interactions and post-transcriptional regulatory networks.

Non-coding RNA Regulation

Circular RNA profiles can support studies of non-coding RNA regulation, including circRNA–miRNA interactions and post-transcriptional regulatory networks.

Disease and Biomarker Research

circRNA-seq can support research into disease mechanisms and biomarker discovery by identifying circRNA expression patterns linked to biological or clinical phenotypes.

Disease and Biomarker Research

circRNA-seq can support research into disease mechanisms and biomarker discovery by identifying circRNA expression patterns linked to biological or clinical phenotypes.

Development and Cell State Studies

circRNA-seq supports studies of development, differentiation and cell-state changes by comparing circular RNA expression across stages, tissues or experimental conditions.

Development and Cell State Studies

circRNA-seq supports studies of development, differentiation and cell-state changes by comparing circular RNA expression across stages, tissues or experimental conditions.

Applications

Regulatory Response Analysis

circRNA-seq helps explore circular RNA changes linked to development, disease, treatment response or cellular adaptation across different biological conditions.

Regulatory Response Analysis

circRNA-seq helps explore circular RNA changes linked to development, disease, treatment response or cellular adaptation across different biological conditions.

Non-coding RNA Regulation

Circular RNA profiles can support studies of non-coding RNA regulation, including circRNA–miRNA interactions and post-transcriptional regulatory networks.

Non-coding RNA Regulation

Circular RNA profiles can support studies of non-coding RNA regulation, including circRNA–miRNA interactions and post-transcriptional regulatory networks.

Disease and Biomarker Research

circRNA-seq can support research into disease mechanisms and biomarker discovery by identifying circRNA expression patterns linked to biological or clinical phenotypes.

Disease and Biomarker Research

circRNA-seq can support research into disease mechanisms and biomarker discovery by identifying circRNA expression patterns linked to biological or clinical phenotypes.

Development and Cell State Studies

circRNA-seq supports studies of development, differentiation and cell-state changes by comparing circular RNA expression across stages, tissues or experimental conditions.

Development and Cell State Studies

circRNA-seq supports studies of development, differentiation and cell-state changes by comparing circular RNA expression across stages, tissues or experimental conditions.

Specifications

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity Number (Agilent 5400)Purity (NanoDrop)
circRNA LibraryTotal RNA≥ 1 μgAnimal ≥ 7, Plant ≥ 6.5, with smooth baselineOD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity Number (Agilent 5400)Purity (NanoDrop)
circRNA LibraryTotal RNA≥ 1 μgAnimal ≥ 7, Plant ≥ 6.5, with smooth baselineOD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity Number (Agilent 5400)Purity (NanoDrop)
circRNA LibraryTotal RNA≥ 1 μgAnimal ≥ 7, Plant ≥ 6.5, with smooth baselineOD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity Number (Agilent 5400)Purity (NanoDrop)
circRNA LibraryTotal RNA≥ 1 μgAnimal ≥ 7, Plant ≥ 6.5, with smooth baselineOD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no contamination

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired-end 150bp
Recommended Sequencing Depth≥ 40 million read pair per sample
Standard Data AnalysisData Quality Control
circRNA Identification
Transcript Expression Quantification & Differential Expression Analysis
Functional Enrichment Analysis
CircRNA Target Gene Prediction

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired-end 150bp
Recommended Sequencing Depth≥ 40 million read pair per sample
Standard Data AnalysisData Quality Control
circRNA Identification
Transcript Expression Quantification & Differential Expression Analysis
Functional Enrichment Analysis
CircRNA Target Gene Prediction

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired-end 150bp
Recommended Sequencing Depth≥ 40 million read pair per sample
Standard Data AnalysisData Quality Control
circRNA Identification
Transcript Expression Quantification & Differential Expression Analysis
Functional Enrichment Analysis
CircRNA Target Gene Prediction

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired-end 150bp
Recommended Sequencing Depth≥ 40 million read pair per sample
Standard Data AnalysisData Quality Control
circRNA Identification
Transcript Expression Quantification & Differential Expression Analysis
Functional Enrichment Analysis
CircRNA Target Gene Prediction

Specifications

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity Number (Agilent 5400)Purity (NanoDrop)
circRNA LibraryTotal RNA≥ 1 μgAnimal ≥ 7, Plant ≥ 6.5, with smooth baselineOD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity Number (Agilent 5400)Purity (NanoDrop)
circRNA LibraryTotal RNA≥ 1 μgAnimal ≥ 7, Plant ≥ 6.5, with smooth baselineOD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity Number (Agilent 5400)Purity (NanoDrop)
circRNA LibraryTotal RNA≥ 1 μgAnimal ≥ 7, Plant ≥ 6.5, with smooth baselineOD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity Number (Agilent 5400)Purity (NanoDrop)
circRNA LibraryTotal RNA≥ 1 μgAnimal ≥ 7, Plant ≥ 6.5, with smooth baselineOD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no contamination

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired-end 150bp
Recommended Sequencing Depth≥ 40 million read pair per sample
Standard Data AnalysisData Quality Control
circRNA Identification
Transcript Expression Quantification & Differential Expression Analysis
Functional Enrichment Analysis
CircRNA Target Gene Prediction

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired-end 150bp
Recommended Sequencing Depth≥ 40 million read pair per sample
Standard Data AnalysisData Quality Control
circRNA Identification
Transcript Expression Quantification & Differential Expression Analysis
Functional Enrichment Analysis
CircRNA Target Gene Prediction

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired-end 150bp
Recommended Sequencing Depth≥ 40 million read pair per sample
Standard Data AnalysisData Quality Control
circRNA Identification
Transcript Expression Quantification & Differential Expression Analysis
Functional Enrichment Analysis
CircRNA Target Gene Prediction

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired-end 150bp
Recommended Sequencing Depth≥ 40 million read pair per sample
Standard Data AnalysisData Quality Control
circRNA Identification
Transcript Expression Quantification & Differential Expression Analysis
Functional Enrichment Analysis
CircRNA Target Gene Prediction

Resources

Example Analysis Output

Image
Image
1/1
circRNA Genome Distribution

Visualise circRNA density and distribution across chromosomes using a Circos plot.

Image
Image
1/1
circRNA Expression

Review circRNA expression levels across samples using TPM-based expression distribution plots.

Image
Image
1/1
circRNA Volcano Plot

Highlight significantly up- and down-regulated circRNAs based on fold change and statistical significance.

Image
Image
1/1
KEGG Pathway Enrichment

Identify enriched pathways associated with differentially expressed circRNAs to support pathway-level interpretation.

Image
Image
1/1
circRNA Genome Distribution

Visualise circRNA density and distribution across chromosomes using a Circos plot.

Image
Image
1/1
circRNA Expression

Review circRNA expression levels across samples using TPM-based expression distribution plots.

Image
Image
1/1
circRNA Volcano Plot

Highlight significantly up- and down-regulated circRNAs based on fold change and statistical significance.

Image
Image
1/1
KEGG Pathway Enrichment

Identify enriched pathways associated with differentially expressed circRNAs to support pathway-level interpretation.

Image
Image
1/1
circRNA Genome Distribution

Visualise circRNA density and distribution across chromosomes using a Circos plot.

Image
Image
1/1
circRNA Expression

Review circRNA expression levels across samples using TPM-based expression distribution plots.

Image
Image
1/1
circRNA Volcano Plot

Highlight significantly up- and down-regulated circRNAs based on fold change and statistical significance.

Image
Image
1/1
KEGG Pathway Enrichment

Identify enriched pathways associated with differentially expressed circRNAs to support pathway-level interpretation.

Image
Image
1/1
circRNA Genome Distribution

Visualise circRNA density and distribution across chromosomes using a Circos plot.

Image
Image
1/1
circRNA Expression

Review circRNA expression levels across samples using TPM-based expression distribution plots.

Image
Image
1/1
circRNA Volcano Plot

Highlight significantly up- and down-regulated circRNAs based on fold change and statistical significance.

Image
Image
1/1
KEGG Pathway Enrichment

Identify enriched pathways associated with differentially expressed circRNAs to support pathway-level interpretation.

Webinars

ncRNA-seq Results Explained: What you can explore with non-coding RNA data

Learn how to navigate and interpret non-coding RNA sequencing (ncRNA-seq) data, including lncRNA-seq, small RNA-seq, and circRNA-seq. This webinar will provide an overview of analysis workflows, data visualisation approaches, and the tools needed to transform complex datasets into meaningful biological insights.

Learn More
(ncRNA-seq Results Explained: What you can explore with non-coding RNA data)
ncRNA-seq Results Explained: What you can explore with non-coding RNA data

Learn how to navigate and interpret non-coding RNA sequencing (ncRNA-seq) data, including lncRNA-seq, small RNA-seq, and circRNA-seq. This webinar will provide an overview of analysis workflows, data visualisation approaches, and the tools needed to transform complex datasets into meaningful biological insights.

Learn More
(ncRNA-seq Results Explained: What you can explore with non-coding RNA data)
ncRNA-seq Results Explained: What you can explore with non-coding RNA data

Learn how to navigate and interpret non-coding RNA sequencing (ncRNA-seq) data, including lncRNA-seq, small RNA-seq, and circRNA-seq. This webinar will provide an overview of analysis workflows, data visualisation approaches, and the tools needed to transform complex datasets into meaningful biological insights.

Learn More
(ncRNA-seq Results Explained: What you can explore with non-coding RNA data)
ncRNA-seq Results Explained: What you can explore with non-coding RNA data

Learn how to navigate and interpret non-coding RNA sequencing (ncRNA-seq) data, including lncRNA-seq, small RNA-seq, and circRNA-seq. This webinar will provide an overview of analysis workflows, data visualisation approaches, and the tools needed to transform complex datasets into meaningful biological insights.

Learn More
(ncRNA-seq Results Explained: What you can explore with non-coding RNA data)

Frequently Asked Questions

What is circRNA sequencing used for?

circRNA-seq profiles circular RNAs and quantifies their expression. Researchers use it to study biological function and disease relevance.

What are the key differences between circRNA-seq and mRNA-seq?

Can circRNA-seq detect novel circRNAs?

What types of studies are suitable for circRNA-seq?

Does circRNA-seq provide quantitative expression data?

Does Novogene provide bioinformatics analysis for circular RNA profiling?

Can circRNA-seq data be integrated with mRNA-seq or total RNA-seq?

Explore Transcriptomics Insights

Access webinars, brochures and case studies covering sequencing technologies, study planning and data interpretation.
View Webinar Library
(Explore Transcriptomics Insights)
View Webinar Library
(Explore Transcriptomics Insights)
Background
Background

Speak with a Specialist

Have a project in mind?

 

Send us your enquiry and a Novogene Europe specialist will be in touch.

 
 
 
 
 
 
 
 
 
 
Privacy PolicyCookie PolicyTerms
banner laptop bg
banner mobile bg

Circular RNA Sequencing (circRNA-seq)

Profiling circular RNAs to explore back-splice junctions, non-coding RNA regulation and expression changes across biological conditions.
OverviewOverview
BenefitsBenefits
ApplicationsApplications
SpecificationsSpecifications
ResourcesResources

Circular RNA sequencing, also known as circRNA-seq, enables the identification and quantification of circular RNAs generated through back-splicing events. By enriching and sequencing circular transcripts, circRNA-seq helps researchers detect back-splice junctions and compare circRNA expression across samples or experimental conditions.


Novogene Europe supports circRNA-seq projects using qualified total RNA inputs, library preparation workflows designed for circular RNA enrichment, high-throughput sequencing and bioinformatics analysis. The service supports circRNA identification, expression quantification, differential expression analysis, functional enrichment and target gene prediction.

Service Highlights

Circular Transcript Profiling
Circular Transcript Profiling

Profile circular RNAs across samples and conditions to explore non-coding RNA regulation, expression changes and biological responses across different study groups.

Circular Transcript Profiling
Circular Transcript Profiling

Profile circular RNAs across samples and conditions to explore non-coding RNA regulation, expression changes and biological responses across different study groups.

Circular RNA Enrichment Workflow
Circular RNA Enrichment Workflow

Library preparation workflows are designed to enrich circular RNA signals from qualified RNA inputs, supporting downstream sequencing and circRNA-focused analysis.

Circular RNA Enrichment Workflow
Circular RNA Enrichment Workflow

Library preparation workflows are designed to enrich circular RNA signals from qualified RNA inputs, supporting downstream sequencing and circRNA-focused analysis.

Back-splice Junction Identification
Back-splice Junction Identification

Bioinformatics analysis supports detection of back-splice junctions, helping identify circular transcripts and distinguish circRNA signals from linear RNA.

Back-splice Junction Identification
Back-splice Junction Identification

Bioinformatics analysis supports detection of back-splice junctions, helping identify circular transcripts and distinguish circRNA signals from linear RNA.

Regulatory and Functional Insight
Regulatory and Functional Insight

Analyse differential circRNA expression, predicted target interactions and functional enrichment to support biological interpretation of circRNA-associated regulatory changes.

Regulatory and Functional Insight
Regulatory and Functional Insight

Analyse differential circRNA expression, predicted target interactions and functional enrichment to support biological interpretation of circRNA-associated regulatory changes.

Service Highlights

Circular Transcript Profiling
Circular Transcript Profiling

Profile circular RNAs across samples and conditions to explore non-coding RNA regulation, expression changes and biological responses across different study groups.

Circular Transcript Profiling
Circular Transcript Profiling

Profile circular RNAs across samples and conditions to explore non-coding RNA regulation, expression changes and biological responses across different study groups.

Circular RNA Enrichment Workflow
Circular RNA Enrichment Workflow

Library preparation workflows are designed to enrich circular RNA signals from qualified RNA inputs, supporting downstream sequencing and circRNA-focused analysis.

Circular RNA Enrichment Workflow
Circular RNA Enrichment Workflow

Library preparation workflows are designed to enrich circular RNA signals from qualified RNA inputs, supporting downstream sequencing and circRNA-focused analysis.

Back-splice Junction Identification
Back-splice Junction Identification

Bioinformatics analysis supports detection of back-splice junctions, helping identify circular transcripts and distinguish circRNA signals from linear RNA.

Back-splice Junction Identification
Back-splice Junction Identification

Bioinformatics analysis supports detection of back-splice junctions, helping identify circular transcripts and distinguish circRNA signals from linear RNA.

Regulatory and Functional Insight
Regulatory and Functional Insight

Analyse differential circRNA expression, predicted target interactions and functional enrichment to support biological interpretation of circRNA-associated regulatory changes.

Regulatory and Functional Insight
Regulatory and Functional Insight

Analyse differential circRNA expression, predicted target interactions and functional enrichment to support biological interpretation of circRNA-associated regulatory changes.

Applications

Regulatory Response Analysis

circRNA-seq helps explore circular RNA changes linked to development, disease, treatment response or cellular adaptation across different biological conditions.

Regulatory Response Analysis

circRNA-seq helps explore circular RNA changes linked to development, disease, treatment response or cellular adaptation across different biological conditions.

Non-coding RNA Regulation

Circular RNA profiles can support studies of non-coding RNA regulation, including circRNA–miRNA interactions and post-transcriptional regulatory networks.

Non-coding RNA Regulation

Circular RNA profiles can support studies of non-coding RNA regulation, including circRNA–miRNA interactions and post-transcriptional regulatory networks.

Disease and Biomarker Research

circRNA-seq can support research into disease mechanisms and biomarker discovery by identifying circRNA expression patterns linked to biological or clinical phenotypes.

Disease and Biomarker Research

circRNA-seq can support research into disease mechanisms and biomarker discovery by identifying circRNA expression patterns linked to biological or clinical phenotypes.

Development and Cell State Studies

circRNA-seq supports studies of development, differentiation and cell-state changes by comparing circular RNA expression across stages, tissues or experimental conditions.

Development and Cell State Studies

circRNA-seq supports studies of development, differentiation and cell-state changes by comparing circular RNA expression across stages, tissues or experimental conditions.

Applications

Regulatory Response Analysis

circRNA-seq helps explore circular RNA changes linked to development, disease, treatment response or cellular adaptation across different biological conditions.

Regulatory Response Analysis

circRNA-seq helps explore circular RNA changes linked to development, disease, treatment response or cellular adaptation across different biological conditions.

Non-coding RNA Regulation

Circular RNA profiles can support studies of non-coding RNA regulation, including circRNA–miRNA interactions and post-transcriptional regulatory networks.

Non-coding RNA Regulation

Circular RNA profiles can support studies of non-coding RNA regulation, including circRNA–miRNA interactions and post-transcriptional regulatory networks.

Disease and Biomarker Research

circRNA-seq can support research into disease mechanisms and biomarker discovery by identifying circRNA expression patterns linked to biological or clinical phenotypes.

Disease and Biomarker Research

circRNA-seq can support research into disease mechanisms and biomarker discovery by identifying circRNA expression patterns linked to biological or clinical phenotypes.

Development and Cell State Studies

circRNA-seq supports studies of development, differentiation and cell-state changes by comparing circular RNA expression across stages, tissues or experimental conditions.

Development and Cell State Studies

circRNA-seq supports studies of development, differentiation and cell-state changes by comparing circular RNA expression across stages, tissues or experimental conditions.

Specifications

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity Number (Agilent 5400)Purity (NanoDrop)
circRNA LibraryTotal RNA≥ 1 μgAnimal ≥ 7, Plant ≥ 6.5, with smooth baselineOD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity Number (Agilent 5400)Purity (NanoDrop)
circRNA LibraryTotal RNA≥ 1 μgAnimal ≥ 7, Plant ≥ 6.5, with smooth baselineOD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity Number (Agilent 5400)Purity (NanoDrop)
circRNA LibraryTotal RNA≥ 1 μgAnimal ≥ 7, Plant ≥ 6.5, with smooth baselineOD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity Number (Agilent 5400)Purity (NanoDrop)
circRNA LibraryTotal RNA≥ 1 μgAnimal ≥ 7, Plant ≥ 6.5, with smooth baselineOD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no contamination

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired-end 150bp
Recommended Sequencing Depth≥ 40 million read pair per sample
Standard Data AnalysisData Quality Control
circRNA Identification
Transcript Expression Quantification & Differential Expression Analysis
Functional Enrichment Analysis
CircRNA Target Gene Prediction

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired-end 150bp
Recommended Sequencing Depth≥ 40 million read pair per sample
Standard Data AnalysisData Quality Control
circRNA Identification
Transcript Expression Quantification & Differential Expression Analysis
Functional Enrichment Analysis
CircRNA Target Gene Prediction

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired-end 150bp
Recommended Sequencing Depth≥ 40 million read pair per sample
Standard Data AnalysisData Quality Control
circRNA Identification
Transcript Expression Quantification & Differential Expression Analysis
Functional Enrichment Analysis
CircRNA Target Gene Prediction

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired-end 150bp
Recommended Sequencing Depth≥ 40 million read pair per sample
Standard Data AnalysisData Quality Control
circRNA Identification
Transcript Expression Quantification & Differential Expression Analysis
Functional Enrichment Analysis
CircRNA Target Gene Prediction

Specifications

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity Number (Agilent 5400)Purity (NanoDrop)
circRNA LibraryTotal RNA≥ 1 μgAnimal ≥ 7, Plant ≥ 6.5, with smooth baselineOD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity Number (Agilent 5400)Purity (NanoDrop)
circRNA LibraryTotal RNA≥ 1 μgAnimal ≥ 7, Plant ≥ 6.5, with smooth baselineOD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity Number (Agilent 5400)Purity (NanoDrop)
circRNA LibraryTotal RNA≥ 1 μgAnimal ≥ 7, Plant ≥ 6.5, with smooth baselineOD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity Number (Agilent 5400)Purity (NanoDrop)
circRNA LibraryTotal RNA≥ 1 μgAnimal ≥ 7, Plant ≥ 6.5, with smooth baselineOD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no contamination

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired-end 150bp
Recommended Sequencing Depth≥ 40 million read pair per sample
Standard Data AnalysisData Quality Control
circRNA Identification
Transcript Expression Quantification & Differential Expression Analysis
Functional Enrichment Analysis
CircRNA Target Gene Prediction

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired-end 150bp
Recommended Sequencing Depth≥ 40 million read pair per sample
Standard Data AnalysisData Quality Control
circRNA Identification
Transcript Expression Quantification & Differential Expression Analysis
Functional Enrichment Analysis
CircRNA Target Gene Prediction

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired-end 150bp
Recommended Sequencing Depth≥ 40 million read pair per sample
Standard Data AnalysisData Quality Control
circRNA Identification
Transcript Expression Quantification & Differential Expression Analysis
Functional Enrichment Analysis
CircRNA Target Gene Prediction

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina NovaSeq System
Read LengthPaired-end 150bp
Recommended Sequencing Depth≥ 40 million read pair per sample
Standard Data AnalysisData Quality Control
circRNA Identification
Transcript Expression Quantification & Differential Expression Analysis
Functional Enrichment Analysis
CircRNA Target Gene Prediction

Resources

Example Analysis Output

Image
Image
1/1
circRNA Genome Distribution

Visualise circRNA density and distribution across chromosomes using a Circos plot.

Image
Image
1/1
circRNA Expression

Review circRNA expression levels across samples using TPM-based expression distribution plots.

Image
Image
1/1
circRNA Volcano Plot

Highlight significantly up- and down-regulated circRNAs based on fold change and statistical significance.

Image
Image
1/1
KEGG Pathway Enrichment

Identify enriched pathways associated with differentially expressed circRNAs to support pathway-level interpretation.

Image
Image
1/1
circRNA Genome Distribution

Visualise circRNA density and distribution across chromosomes using a Circos plot.

Image
Image
1/1
circRNA Expression

Review circRNA expression levels across samples using TPM-based expression distribution plots.

Image
Image
1/1
circRNA Volcano Plot

Highlight significantly up- and down-regulated circRNAs based on fold change and statistical significance.

Image
Image
1/1
KEGG Pathway Enrichment

Identify enriched pathways associated with differentially expressed circRNAs to support pathway-level interpretation.

Image
Image
1/1
circRNA Genome Distribution

Visualise circRNA density and distribution across chromosomes using a Circos plot.

Image
Image
1/1
circRNA Expression

Review circRNA expression levels across samples using TPM-based expression distribution plots.

Image
Image
1/1
circRNA Volcano Plot

Highlight significantly up- and down-regulated circRNAs based on fold change and statistical significance.

Image
Image
1/1
KEGG Pathway Enrichment

Identify enriched pathways associated with differentially expressed circRNAs to support pathway-level interpretation.

Image
Image
1/1
circRNA Genome Distribution

Visualise circRNA density and distribution across chromosomes using a Circos plot.

Image
Image
1/1
circRNA Expression

Review circRNA expression levels across samples using TPM-based expression distribution plots.

Image
Image
1/1
circRNA Volcano Plot

Highlight significantly up- and down-regulated circRNAs based on fold change and statistical significance.

Image
Image
1/1
KEGG Pathway Enrichment

Identify enriched pathways associated with differentially expressed circRNAs to support pathway-level interpretation.

Webinars

ncRNA-seq Results Explained: What you can explore with non-coding RNA data

Learn how to navigate and interpret non-coding RNA sequencing (ncRNA-seq) data, including lncRNA-seq, small RNA-seq, and circRNA-seq. This webinar will provide an overview of analysis workflows, data visualisation approaches, and the tools needed to transform complex datasets into meaningful biological insights.

Learn More
(ncRNA-seq Results Explained: What you can explore with non-coding RNA data)
ncRNA-seq Results Explained: What you can explore with non-coding RNA data

Learn how to navigate and interpret non-coding RNA sequencing (ncRNA-seq) data, including lncRNA-seq, small RNA-seq, and circRNA-seq. This webinar will provide an overview of analysis workflows, data visualisation approaches, and the tools needed to transform complex datasets into meaningful biological insights.

Learn More
(ncRNA-seq Results Explained: What you can explore with non-coding RNA data)
ncRNA-seq Results Explained: What you can explore with non-coding RNA data

Learn how to navigate and interpret non-coding RNA sequencing (ncRNA-seq) data, including lncRNA-seq, small RNA-seq, and circRNA-seq. This webinar will provide an overview of analysis workflows, data visualisation approaches, and the tools needed to transform complex datasets into meaningful biological insights.

Learn More
(ncRNA-seq Results Explained: What you can explore with non-coding RNA data)
ncRNA-seq Results Explained: What you can explore with non-coding RNA data

Learn how to navigate and interpret non-coding RNA sequencing (ncRNA-seq) data, including lncRNA-seq, small RNA-seq, and circRNA-seq. This webinar will provide an overview of analysis workflows, data visualisation approaches, and the tools needed to transform complex datasets into meaningful biological insights.

Learn More
(ncRNA-seq Results Explained: What you can explore with non-coding RNA data)

Frequently Asked Questions

What is circRNA sequencing used for?

circRNA-seq profiles circular RNAs and quantifies their expression. Researchers use it to study biological function and disease relevance.

What are the key differences between circRNA-seq and mRNA-seq?

Can circRNA-seq detect novel circRNAs?

What types of studies are suitable for circRNA-seq?

Does circRNA-seq provide quantitative expression data?

Does Novogene provide bioinformatics analysis for circular RNA profiling?

Can circRNA-seq data be integrated with mRNA-seq or total RNA-seq?

Explore Transcriptomics Insights

Access webinars, brochures and case studies covering sequencing technologies, study planning and data interpretation.
View Webinar Library
(Explore Transcriptomics Insights)
View Webinar Library
(Explore Transcriptomics Insights)
Background
Background

Speak with a Specialist

Have a project in mind?

 

Send us your enquiry and a Novogene Europe specialist will be in touch.

 
 
 
 
 
 
 
 
 
 
Privacy PolicyCookie PolicyTerms