Plant and Animal Whole Genome Sequencing

Whole genome sequencing for plant and animal genetic variation, population studies, breeding research and evolutionary genomics.

Plant and animal whole genome sequencing provides a genome-wide view of genetic variation across model and non-model species. It supports the detection of SNPs, indels, copy number variants and structural variants, helping researchers study traits, diversity, population structure and genome evolution.

Novogene Europe supports plant and animal WGS projects across a wide range of species and research goals. With short-read and long-read sequencing options, established quality control and bioinformatics workflows, we help researchers investigate genome-wide variation in breeding, population genetics, evolutionary biology and trait-related studies.

Service Highlights

Broad Species Support

Support whole genome sequencing projects across plant and animal species, including model organisms, agricultural species and non-model genomes.

Genome Variation Analysis

Detect SNPs, indels, copy number variants and structural variants to study genetic diversity, trait-associated variation, adaptation and genome evolution.

Flexible Sequencing Strategies

Use short-read or long-read sequencing strategies to match genome complexity, study scale and the level of variant resolution required.

Population and Breeding Insight

Support comparative analysis across populations, breeds, accessions or experimental groups for breeding research, GWAS and population genomics.

Applications

Population Genetics

Plant and animal WGS supports comparison of genome-wide variation across populations, breeds, accessions or lineages to study genetic diversity and population structure.

Trait and GWAS Studies

Genome-wide variant data can support association studies, helping researchers investigate genetic markers linked to important biological, agricultural or phenotypic traits.

Breeding Research

Whole genome sequencing can support breeding research by identifying genetic variation relevant to selection, improvement, diversity management and lineage tracking.

Evolution and Adaptation

Plant and animal WGS helps researchers study genome evolution, adaptation and species differentiation across diverse environmental, ecological or domestication contexts.

Specifications

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	Purity(NanoDrop™/Agarose Gel)
Plant & Animal WGS (350 bp insert size)	Genomic DNA	≥100 ng	≥20 μL	≥5 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination Fragments longer than 1000 bp
PCR-freePlant & Animal WGS (350 bp insert size)	Genomic DNA	≥1.2 μg	≥20 μL	≥20 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination
PCR-freePlant & Animal WGS (custom size, ≤500 bp)	Genomic DNA	≥2 μg	≥20 μL	≥30 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	Purity(NanoDrop™/Agarose Gel)
Plant & Animal WGS (350 bp insert size)	Genomic DNA	≥100 ng	≥20 μL	≥5 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination Fragments longer than 1000 bp
PCR-freePlant & Animal WGS (350 bp insert size)	Genomic DNA	≥1.2 μg	≥20 μL	≥20 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination
PCR-freePlant & Animal WGS (custom size, ≤500 bp)	Genomic DNA	≥2 μg	≥20 μL	≥30 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	Purity(NanoDrop™/Agarose Gel)
Plant & Animal WGS (350 bp insert size)	Genomic DNA	≥100 ng	≥20 μL	≥5 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination Fragments longer than 1000 bp
PCR-freePlant & Animal WGS (350 bp insert size)	Genomic DNA	≥1.2 μg	≥20 μL	≥20 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination
PCR-freePlant & Animal WGS (custom size, ≤500 bp)	Genomic DNA	≥2 μg	≥20 μL	≥30 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	Purity(NanoDrop™/Agarose Gel)
Plant & Animal WGS (350 bp insert size)	Genomic DNA	≥100 ng	≥20 μL	≥5 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination Fragments longer than 1000 bp
PCR-freePlant & Animal WGS (350 bp insert size)	Genomic DNA	≥1.2 μg	≥20 μL	≥20 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination
PCR-freePlant & Animal WGS (custom size, ≤500 bp)	Genomic DNA	≥2 μg	≥20 μL	≥30 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis Data quality controlSequencing error rateFiltering reads containing adapter or with low qualityAlignment with reference genomeStatistics of mapping, sequencing depth and coverageSNP calling, annotation and statistics Advanced Analysis SV calling, annotation and statisticsCNV calling, annotation and statistics	Standard Analysis Data quality control Sequencing alignment Structural Variant (SV) detection

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis Data quality controlSequencing error rateFiltering reads containing adapter or with low qualityAlignment with reference genomeStatistics of mapping, sequencing depth and coverageSNP calling, annotation and statistics Advanced Analysis SV calling, annotation and statisticsCNV calling, annotation and statistics	Standard Analysis Data quality control Sequencing alignment Structural Variant (SV) detection

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis Data quality controlSequencing error rateFiltering reads containing adapter or with low qualityAlignment with reference genomeStatistics of mapping, sequencing depth and coverageSNP calling, annotation and statistics Advanced Analysis SV calling, annotation and statisticsCNV calling, annotation and statistics	Standard Analysis Data quality control Sequencing alignment Structural Variant (SV) detection

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis Data quality controlSequencing error rateFiltering reads containing adapter or with low qualityAlignment with reference genomeStatistics of mapping, sequencing depth and coverageSNP calling, annotation and statistics Advanced Analysis SV calling, annotation and statisticsCNV calling, annotation and statistics	Standard Analysis Data quality control Sequencing alignment Structural Variant (SV) detection

Specifications

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	Purity(NanoDrop™/Agarose Gel)
Plant & Animal WGS (350 bp insert size)	Genomic DNA	≥100 ng	≥20 μL	≥5 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination Fragments longer than 1000 bp
PCR-freePlant & Animal WGS (350 bp insert size)	Genomic DNA	≥1.2 μg	≥20 μL	≥20 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination
PCR-freePlant & Animal WGS (custom size, ≤500 bp)	Genomic DNA	≥2 μg	≥20 μL	≥30 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	Purity(NanoDrop™/Agarose Gel)
Plant & Animal WGS (350 bp insert size)	Genomic DNA	≥100 ng	≥20 μL	≥5 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination Fragments longer than 1000 bp
PCR-freePlant & Animal WGS (350 bp insert size)	Genomic DNA	≥1.2 μg	≥20 μL	≥20 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination
PCR-freePlant & Animal WGS (custom size, ≤500 bp)	Genomic DNA	≥2 μg	≥20 μL	≥30 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	Purity(NanoDrop™/Agarose Gel)
Plant & Animal WGS (350 bp insert size)	Genomic DNA	≥100 ng	≥20 μL	≥5 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination Fragments longer than 1000 bp
PCR-freePlant & Animal WGS (350 bp insert size)	Genomic DNA	≥1.2 μg	≥20 μL	≥20 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination
PCR-freePlant & Animal WGS (custom size, ≤500 bp)	Genomic DNA	≥2 μg	≥20 μL	≥30 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Service	Sample Type	Amount (Qubit®)	Volume	Concentration	Purity(NanoDrop™/Agarose Gel)
Plant & Animal WGS (350 bp insert size)	Genomic DNA	≥100 ng	≥20 μL	≥5 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination Fragments longer than 1000 bp
PCR-freePlant & Animal WGS (350 bp insert size)	Genomic DNA	≥1.2 μg	≥20 μL	≥20 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination
PCR-freePlant & Animal WGS (custom size, ≤500 bp)	Genomic DNA	≥2 μg	≥20 μL	≥30 ng/μL	OD260/280 = 1.8-2.0; no degradation, no contamination

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis Data quality controlSequencing error rateFiltering reads containing adapter or with low qualityAlignment with reference genomeStatistics of mapping, sequencing depth and coverageSNP calling, annotation and statistics Advanced Analysis SV calling, annotation and statisticsCNV calling, annotation and statistics	Standard Analysis Data quality control Sequencing alignment Structural Variant (SV) detection

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis Data quality controlSequencing error rateFiltering reads containing adapter or with low qualityAlignment with reference genomeStatistics of mapping, sequencing depth and coverageSNP calling, annotation and statistics Advanced Analysis SV calling, annotation and statisticsCNV calling, annotation and statistics	Standard Analysis Data quality control Sequencing alignment Structural Variant (SV) detection

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis Data quality controlSequencing error rateFiltering reads containing adapter or with low qualityAlignment with reference genomeStatistics of mapping, sequencing depth and coverageSNP calling, annotation and statistics Advanced Analysis SV calling, annotation and statisticsCNV calling, annotation and statistics	Standard Analysis Data quality control Sequencing alignment Structural Variant (SV) detection

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Platform Type	Illumina NovaSeq System	PacBio Revio System	Nanopore PromethION
Read Length	Paired-end 150 bp	Average > 15 kb	Average > 17 kb
Recommended Sequencing Depth	For SNP/InDel detection: ≥ 10×	For SV detection: ≥ 20×
Recommended Sequencing Depth	For SV/CNV detection: ≥ 20×	For SV detection: ≥ 20×
Content of Analysis	Standard Analysis Data quality controlSequencing error rateFiltering reads containing adapter or with low qualityAlignment with reference genomeStatistics of mapping, sequencing depth and coverageSNP calling, annotation and statistics Advanced Analysis SV calling, annotation and statisticsCNV calling, annotation and statistics	Standard Analysis Data quality control Sequencing alignment Structural Variant (SV) detection

Resources

Example Analysis Output

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Mutation Spectrum

Compare the proportions of different mutation types across samples to summarise overall variant patterns.

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Chromosome Density

Visualise variant density across chromosomes to identify distribution patterns and regions of enrichment.

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Genome-wide Variant Map

Use a Circos plot to summarise SNPs, indels and structural variants across the genome.

Webinars

Getting Started with Genomics: Next Generation Sequencing Essentials for 2024

Discover the fundamentals of Next-Generation Sequencing (NGS) and learn how leading technologies such as Illumina, PacBio, and Oxford Nanopore are advancing genomic research and unlocking new possibilities in DNA analysis.

Learn More

Frequently Asked Questions

What types of organisms are suitable for Plant and animal whole genome sequencing?

Plant and animal whole genome sequencing supports a wide range of plant and animal species, including model organisms, agricultural species and non-model organisms.

What sequencing strategies does Plant and animal whole genome sequencing use?

What types of genetic variants can Plant and animal whole genome sequencing detect?

When should researchers choose long-read sequencing?

How does Plant and animal whole genome sequencing differ from de novo genome sequencing?

What sample quality does Plant and animal whole genome sequencing require?

Does Plant and animal whole genome sequencing support population or comparative studies?

Does Novogene provide support for analysing Plant and animal whole genome sequencing data?

Explore Genomics Insights

Access webinars, brochures and case studies covering sequencing technologies, study planning and data interpretation.

Speak with a Specialist

Have a project in mind?

Send us your enquiry and a Novogene Europe specialist will be in touch.