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Human Whole Genome SequencingWhole Exome SequencingPlant and Animal Whole Genome SequencingPlant and Animal De novo SequencingDNA Methylation SequencingmRNA SequencingFull-Length Transcriptome SequencingWhole Transcriptome SequencingMetatranscriptome SequencingShotgun Metagenomics SequencingAmplicon SequencingWhole Plasmid Sequencing10X Single Cell Gene Expression10X Single Cell Immune Profiling10X Visium HD Spatial Gene ExpressionOlink ProteomicsUntargeted MetabolomicsAccredited & Validated Clinical Research Sequencing
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Novogene Europe
  • Novogene Europe
  • Genomics
    • Human Whole Genome Sequencing
    • Whole Exome Sequencing
    • Plant and Animal Whole Genome Sequencing
    • Plant and Animal De novo Sequencing
    • Microbial Whole Genome Sequencing
    • Microbial De novo Sequencing
    • Whole Plasmid SequencingOrder Online!
    Proteomics
    • Olink ProteomicsNew!
    Epigenomics
    • DNA Methylation SequencingUpdated!
    • Chromatin Immunoprecipitation Sequencing (ChIP-seq)
    • RNA Immunoprecipitation Sequencing (RIP-seq)
    Metabolomics
    • Untargeted MetabolomicsComing Soon!
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    • mRNA Sequencing
    • Small RNA Sequencing (sRNA‑seq)
    • Circular RNA Sequencing (circRNA-seq)
    • Total RNA Sequencing
    • Whole Transcriptome Sequencing
    • Full-Length Transcriptome Sequencing
    • Prokaryotic RNA Sequencing
    • Metatranscriptome Sequencing
    Metagenomics
    • Amplicon SequencingOrder Online!
    • Shotgun Metagenomics Sequencing
    Single Cell & Spatial Omics
    • 10X Single Cell Gene Expression
    • 10X Single Cell Immune Profiling
    • 10X Visium HD Spatial Gene Expression
    Premade Library
    • Sequencing Only on Illumina® Sequencer
    • Sequencing Only on Element SequencerNew!
    • Sequencing Only on PacBio Sequencer
    Translational Research
    • Accredited & Validated Clinical Research Sequencing
  • PromotionsPromotions
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    • Automated Delivery Platform (Falcon)
    • Bioinformatics Analysis Tool (NovoMagic)
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    • Immuno-oncology
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    • Rare and Complex Diseases
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  • Contact UsContact Us
    • Amplicon Sequencing
    • Whole Plasmid Sequencing

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Human Whole Genome SequencingWhole Exome SequencingPlant and Animal Whole Genome SequencingPlant and Animal De novo SequencingDNA Methylation SequencingmRNA SequencingFull-Length Transcriptome SequencingWhole Transcriptome SequencingMetatranscriptome SequencingShotgun Metagenomics SequencingAmplicon SequencingWhole Plasmid Sequencing10X Single Cell Gene Expression10X Single Cell Immune Profiling10X Visium HD Spatial Gene ExpressionOlink ProteomicsUntargeted MetabolomicsAccredited & Validated Clinical Research Sequencing
Resources
WebinarsCase StudyBlogBrochure
Support
PlatformBioinformatics Analysis Tool (NovoMagic)Customer Service System (CSS)Customer Support
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Copyright © 2026 Novogene Inc. All rights reserved.For Research Use Only. Not for Clinical Diagnostic Use.
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Microbial De novo Sequencing

Genome assembly and reference genome construction for novel, less-characterised and complex microbial genomes.
OverviewOverview
BenefitsBenefits
ApplicationsApplications
SpecificationsSpecifications
ResourcesResources

Microbial de novo sequencing enables genome assembly without relying on an existing reference genome. It is suitable for novel or less-characterised microorganisms, helping researchers construct draft or high-contiguity genomes and explore genome structure, gene content and functional potential.


Novogene Europe supports microbial de novo sequencing projects with short-read and long-read sequencing strategies, quality control and bioinformatics workflows. The service helps researchers generate reliable microbial genome assemblies for comparative genomics, evolutionary studies and applied microbiology research.

Service Highlights

Reference-free Assembly
Reference-free Assembly

Assemble microbial genomes without an existing reference, supporting genome construction for novel, less-characterised or newly isolated microorganisms.

Reference-free Assembly
Reference-free Assembly

Assemble microbial genomes without an existing reference, supporting genome construction for novel, less-characterised or newly isolated microorganisms.

Flexible Sequencing Strategies
Flexible Sequencing Strategies

Short- or long-read sequencing can be selected based on microbial genome size, sample quality and assembly goals, supporting draft assemblies or higher-contiguity genome reconstruction.

Flexible Sequencing Strategies
Flexible Sequencing Strategies

Short- or long-read sequencing can be selected based on microbial genome size, sample quality and assembly goals, supporting draft assemblies or higher-contiguity genome reconstruction.

Assembly Quality Assessment
Assembly Quality Assessment

Evaluate genome assembly outputs using key metrics such as coverage, contiguity and completeness to support reliable downstream analysis.

Assembly Quality Assessment
Assembly Quality Assessment

Evaluate genome assembly outputs using key metrics such as coverage, contiguity and completeness to support reliable downstream analysis.

Genome Annotation Support
Genome Annotation Support

Bioinformatics workflows can support gene prediction, functional annotation and comparative analysis to help characterise microbial genome resources.

Genome Annotation Support
Genome Annotation Support

Bioinformatics workflows can support gene prediction, functional annotation and comparative analysis to help characterise microbial genome resources.

Service Highlights

Reference-free Assembly
Reference-free Assembly

Assemble microbial genomes without an existing reference, supporting genome construction for novel, less-characterised or newly isolated microorganisms.

Reference-free Assembly
Reference-free Assembly

Assemble microbial genomes without an existing reference, supporting genome construction for novel, less-characterised or newly isolated microorganisms.

Flexible Sequencing Strategies
Flexible Sequencing Strategies

Short- or long-read sequencing can be selected based on microbial genome size, sample quality and assembly goals, supporting draft assemblies or higher-contiguity genome reconstruction.

Flexible Sequencing Strategies
Flexible Sequencing Strategies

Short- or long-read sequencing can be selected based on microbial genome size, sample quality and assembly goals, supporting draft assemblies or higher-contiguity genome reconstruction.

Assembly Quality Assessment
Assembly Quality Assessment

Evaluate genome assembly outputs using key metrics such as coverage, contiguity and completeness to support reliable downstream analysis.

Assembly Quality Assessment
Assembly Quality Assessment

Evaluate genome assembly outputs using key metrics such as coverage, contiguity and completeness to support reliable downstream analysis.

Genome Annotation Support
Genome Annotation Support

Bioinformatics workflows can support gene prediction, functional annotation and comparative analysis to help characterise microbial genome resources.

Genome Annotation Support
Genome Annotation Support

Bioinformatics workflows can support gene prediction, functional annotation and comparative analysis to help characterise microbial genome resources.

Applications

Novel Microorganism Research

Microbial de novo sequencing supports genome analysis for novel or less-characterised microorganisms where a suitable reference genome may not be available.

Novel Microorganism Research

Microbial de novo sequencing supports genome analysis for novel or less-characterised microorganisms where a suitable reference genome may not be available.

Genome Resource Development

De novo assembly can help researchers generate draft or high-contiguity genome resources for downstream annotation, comparison and future genomic studies.

Genome Resource Development

De novo assembly can help researchers generate draft or high-contiguity genome resources for downstream annotation, comparison and future genomic studies.

Functional Genome Exploration

Assembled microbial genomes support gene prediction and functional annotation, helping researchers explore metabolism, adaptation and biological potential.

Functional Genome Exploration

Assembled microbial genomes support gene prediction and functional annotation, helping researchers explore metabolism, adaptation and biological potential.

Evolutionary and Ecological Studies

Microbial genome assemblies can support studies of genome evolution, microbial diversity and adaptation across environmental, agricultural or ecological contexts.

Evolutionary and Ecological Studies

Microbial genome assemblies can support studies of genome evolution, microbial diversity and adaptation across environmental, agricultural or ecological contexts.

Applications

Novel Microorganism Research

Microbial de novo sequencing supports genome analysis for novel or less-characterised microorganisms where a suitable reference genome may not be available.

Novel Microorganism Research

Microbial de novo sequencing supports genome analysis for novel or less-characterised microorganisms where a suitable reference genome may not be available.

Genome Resource Development

De novo assembly can help researchers generate draft or high-contiguity genome resources for downstream annotation, comparison and future genomic studies.

Genome Resource Development

De novo assembly can help researchers generate draft or high-contiguity genome resources for downstream annotation, comparison and future genomic studies.

Functional Genome Exploration

Assembled microbial genomes support gene prediction and functional annotation, helping researchers explore metabolism, adaptation and biological potential.

Functional Genome Exploration

Assembled microbial genomes support gene prediction and functional annotation, helping researchers explore metabolism, adaptation and biological potential.

Evolutionary and Ecological Studies

Microbial genome assemblies can support studies of genome evolution, microbial diversity and adaptation across environmental, agricultural or ecological contexts.

Evolutionary and Ecological Studies

Microbial genome assemblies can support studies of genome evolution, microbial diversity and adaptation across environmental, agricultural or ecological contexts.

Specifications

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

ServiceSample TypeAmount (Qubit®)VolumeConcentrationPurity (NanoDrop™/Agarose Gel)
Microbial de novo sequencingGenomic DNA/ total DNA*≥100 ng≥20 μL≥5 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination Fragments
longer than 1000 bp
PCR-free WGSGenomic DNA / total DNA≥1.2 μg≥20 μL≥20 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination
*Total DNA includes all types of DNA present in a sample, including genomic DNA, mitochondrial DNA and any other extrachromosomal DNA.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

ServiceSample TypeAmount (Qubit®)VolumeConcentrationPurity (NanoDrop™/Agarose Gel)
Microbial de novo sequencingGenomic DNA/ total DNA*≥100 ng≥20 μL≥5 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination Fragments
longer than 1000 bp
PCR-free WGSGenomic DNA / total DNA≥1.2 μg≥20 μL≥20 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination
*Total DNA includes all types of DNA present in a sample, including genomic DNA, mitochondrial DNA and any other extrachromosomal DNA.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

ServiceSample TypeAmount (Qubit®)VolumeConcentrationPurity (NanoDrop™/Agarose Gel)
Microbial de novo sequencingGenomic DNA/ total DNA*≥100 ng≥20 μL≥5 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination Fragments
longer than 1000 bp
PCR-free WGSGenomic DNA / total DNA≥1.2 μg≥20 μL≥20 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination
*Total DNA includes all types of DNA present in a sample, including genomic DNA, mitochondrial DNA and any other extrachromosomal DNA.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

ServiceSample TypeAmount (Qubit®)VolumeConcentrationPurity (NanoDrop™/Agarose Gel)
Microbial de novo sequencingGenomic DNA/ total DNA*≥100 ng≥20 μL≥5 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination Fragments
longer than 1000 bp
PCR-free WGSGenomic DNA / total DNA≥1.2 μg≥20 μL≥20 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination
*Total DNA includes all types of DNA present in a sample, including genomic DNA, mitochondrial DNA and any other extrachromosomal DNA.
erviceSample TypeAmount (Qubit®)VolumeConcentrationPurity
PacBio DNA HiFi libraryHMW genomic DNA (Bacteria & Fungus)≥1.5 μg≥30 μL≥50 ng/μLOD260/280=1.75~2.2;
OD260/230=1.3~2.6;
NC/QC=1.00~2.20
Fragments should be ≥20K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
erviceSample TypeAmount (Qubit®)VolumeConcentrationPurity
PacBio DNA HiFi libraryHMW genomic DNA (Bacteria & Fungus)≥1.5 μg≥30 μL≥50 ng/μLOD260/280=1.75~2.2;
OD260/230=1.3~2.6;
NC/QC=1.00~2.20
Fragments should be ≥20K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
erviceSample TypeAmount (Qubit®)VolumeConcentrationPurity
PacBio DNA HiFi libraryHMW genomic DNA (Bacteria & Fungus)≥1.5 μg≥30 μL≥50 ng/μLOD260/280=1.75~2.2;
OD260/230=1.3~2.6;
NC/QC=1.00~2.20
Fragments should be ≥20K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
erviceSample TypeAmount (Qubit®)VolumeConcentrationPurity
PacBio DNA HiFi libraryHMW genomic DNA (Bacteria & Fungus)≥1.5 μg≥30 μL≥50 ng/μLOD260/280=1.75~2.2;
OD260/230=1.3~2.6;
NC/QC=1.00~2.20
Fragments should be ≥20K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
ServiceSample TypeRecommended Amount VolumeConcentrationPurity
Nanopore PromethION DNA libraryHMW genomic DNA (Bacteria & Fungus)≥3 μg≥50 μL≥60 ng/μLOD260/280=1.7~2.2;
OD260/230=1.3~2.6;
NC/QC=0.95~3.00
Fragments should be ≥15K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
ServiceSample TypeRecommended Amount VolumeConcentrationPurity
Nanopore PromethION DNA libraryHMW genomic DNA (Bacteria & Fungus)≥3 μg≥50 μL≥60 ng/μLOD260/280=1.7~2.2;
OD260/230=1.3~2.6;
NC/QC=0.95~3.00
Fragments should be ≥15K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
ServiceSample TypeRecommended Amount VolumeConcentrationPurity
Nanopore PromethION DNA libraryHMW genomic DNA (Bacteria & Fungus)≥3 μg≥50 μL≥60 ng/μLOD260/280=1.7~2.2;
OD260/230=1.3~2.6;
NC/QC=0.95~3.00
Fragments should be ≥15K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
ServiceSample TypeRecommended Amount VolumeConcentrationPurity
Nanopore PromethION DNA libraryHMW genomic DNA (Bacteria & Fungus)≥3 μg≥50 μL≥60 ng/μLOD260/280=1.7~2.2;
OD260/230=1.3~2.6;
NC/QC=0.95~3.00
Fragments should be ≥15K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Sequel II/IIe System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb(CCS)
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes ≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft MapStandard AnalysisData quality controlGenome preliminarily assemblyGenome component analysis: gene structure prediction, repetitive sequences, non-coding RNAsGene function annotation Bacteria Complete MapStandard AnalysisData quality controlGenome assemblyGenome component analysis Repeat annotation
 Coding gene annotation
 ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
COG  annotation
NR annotation,
TCDB annotation
Pfam annotation
Swiss-Prot annotation
Genome cycle graph
Fungus Fine MapStandard AnalysisData quality controlGenome advanced assemblyGenome surveyGenome component analysisRepeat annotation
Coding gene annotation
ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
NR annotation,
KOG annotation
Pfam annotation
Swiss-Prot annotation.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Sequel II/IIe System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb(CCS)
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes ≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft MapStandard AnalysisData quality controlGenome preliminarily assemblyGenome component analysis: gene structure prediction, repetitive sequences, non-coding RNAsGene function annotation Bacteria Complete MapStandard AnalysisData quality controlGenome assemblyGenome component analysis Repeat annotation
 Coding gene annotation
 ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
COG  annotation
NR annotation,
TCDB annotation
Pfam annotation
Swiss-Prot annotation
Genome cycle graph
Fungus Fine MapStandard AnalysisData quality controlGenome advanced assemblyGenome surveyGenome component analysisRepeat annotation
Coding gene annotation
ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
NR annotation,
KOG annotation
Pfam annotation
Swiss-Prot annotation.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Sequel II/IIe System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb(CCS)
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes ≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft MapStandard AnalysisData quality controlGenome preliminarily assemblyGenome component analysis: gene structure prediction, repetitive sequences, non-coding RNAsGene function annotation Bacteria Complete MapStandard AnalysisData quality controlGenome assemblyGenome component analysis Repeat annotation
 Coding gene annotation
 ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
COG  annotation
NR annotation,
TCDB annotation
Pfam annotation
Swiss-Prot annotation
Genome cycle graph
Fungus Fine MapStandard AnalysisData quality controlGenome advanced assemblyGenome surveyGenome component analysisRepeat annotation
Coding gene annotation
ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
NR annotation,
KOG annotation
Pfam annotation
Swiss-Prot annotation.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Sequel II/IIe System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb(CCS)
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes ≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft MapStandard AnalysisData quality controlGenome preliminarily assemblyGenome component analysis: gene structure prediction, repetitive sequences, non-coding RNAsGene function annotation Bacteria Complete MapStandard AnalysisData quality controlGenome assemblyGenome component analysis Repeat annotation
 Coding gene annotation
 ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
COG  annotation
NR annotation,
TCDB annotation
Pfam annotation
Swiss-Prot annotation
Genome cycle graph
Fungus Fine MapStandard AnalysisData quality controlGenome advanced assemblyGenome surveyGenome component analysisRepeat annotation
Coding gene annotation
ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
NR annotation,
KOG annotation
Pfam annotation
Swiss-Prot annotation.

Specifications

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

ServiceSample TypeAmount (Qubit®)VolumeConcentrationPurity (NanoDrop™/Agarose Gel)
Microbial de novo sequencingGenomic DNA/ total DNA*≥100 ng≥20 μL≥5 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination Fragments
longer than 1000 bp
PCR-free WGSGenomic DNA / total DNA≥1.2 μg≥20 μL≥20 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination
*Total DNA includes all types of DNA present in a sample, including genomic DNA, mitochondrial DNA and any other extrachromosomal DNA.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

ServiceSample TypeAmount (Qubit®)VolumeConcentrationPurity (NanoDrop™/Agarose Gel)
Microbial de novo sequencingGenomic DNA/ total DNA*≥100 ng≥20 μL≥5 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination Fragments
longer than 1000 bp
PCR-free WGSGenomic DNA / total DNA≥1.2 μg≥20 μL≥20 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination
*Total DNA includes all types of DNA present in a sample, including genomic DNA, mitochondrial DNA and any other extrachromosomal DNA.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

ServiceSample TypeAmount (Qubit®)VolumeConcentrationPurity (NanoDrop™/Agarose Gel)
Microbial de novo sequencingGenomic DNA/ total DNA*≥100 ng≥20 μL≥5 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination Fragments
longer than 1000 bp
PCR-free WGSGenomic DNA / total DNA≥1.2 μg≥20 μL≥20 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination
*Total DNA includes all types of DNA present in a sample, including genomic DNA, mitochondrial DNA and any other extrachromosomal DNA.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

ServiceSample TypeAmount (Qubit®)VolumeConcentrationPurity (NanoDrop™/Agarose Gel)
Microbial de novo sequencingGenomic DNA/ total DNA*≥100 ng≥20 μL≥5 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination Fragments
longer than 1000 bp
PCR-free WGSGenomic DNA / total DNA≥1.2 μg≥20 μL≥20 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination
*Total DNA includes all types of DNA present in a sample, including genomic DNA, mitochondrial DNA and any other extrachromosomal DNA.
erviceSample TypeAmount (Qubit®)VolumeConcentrationPurity
PacBio DNA HiFi libraryHMW genomic DNA (Bacteria & Fungus)≥1.5 μg≥30 μL≥50 ng/μLOD260/280=1.75~2.2;
OD260/230=1.3~2.6;
NC/QC=1.00~2.20
Fragments should be ≥20K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
erviceSample TypeAmount (Qubit®)VolumeConcentrationPurity
PacBio DNA HiFi libraryHMW genomic DNA (Bacteria & Fungus)≥1.5 μg≥30 μL≥50 ng/μLOD260/280=1.75~2.2;
OD260/230=1.3~2.6;
NC/QC=1.00~2.20
Fragments should be ≥20K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
erviceSample TypeAmount (Qubit®)VolumeConcentrationPurity
PacBio DNA HiFi libraryHMW genomic DNA (Bacteria & Fungus)≥1.5 μg≥30 μL≥50 ng/μLOD260/280=1.75~2.2;
OD260/230=1.3~2.6;
NC/QC=1.00~2.20
Fragments should be ≥20K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
erviceSample TypeAmount (Qubit®)VolumeConcentrationPurity
PacBio DNA HiFi libraryHMW genomic DNA (Bacteria & Fungus)≥1.5 μg≥30 μL≥50 ng/μLOD260/280=1.75~2.2;
OD260/230=1.3~2.6;
NC/QC=1.00~2.20
Fragments should be ≥20K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
ServiceSample TypeRecommended Amount VolumeConcentrationPurity
Nanopore PromethION DNA libraryHMW genomic DNA (Bacteria & Fungus)≥3 μg≥50 μL≥60 ng/μLOD260/280=1.7~2.2;
OD260/230=1.3~2.6;
NC/QC=0.95~3.00
Fragments should be ≥15K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
ServiceSample TypeRecommended Amount VolumeConcentrationPurity
Nanopore PromethION DNA libraryHMW genomic DNA (Bacteria & Fungus)≥3 μg≥50 μL≥60 ng/μLOD260/280=1.7~2.2;
OD260/230=1.3~2.6;
NC/QC=0.95~3.00
Fragments should be ≥15K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
ServiceSample TypeRecommended Amount VolumeConcentrationPurity
Nanopore PromethION DNA libraryHMW genomic DNA (Bacteria & Fungus)≥3 μg≥50 μL≥60 ng/μLOD260/280=1.7~2.2;
OD260/230=1.3~2.6;
NC/QC=0.95~3.00
Fragments should be ≥15K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
ServiceSample TypeRecommended Amount VolumeConcentrationPurity
Nanopore PromethION DNA libraryHMW genomic DNA (Bacteria & Fungus)≥3 μg≥50 μL≥60 ng/μLOD260/280=1.7~2.2;
OD260/230=1.3~2.6;
NC/QC=0.95~3.00
Fragments should be ≥15K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Sequel II/IIe System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb(CCS)
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes ≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft MapStandard AnalysisData quality controlGenome preliminarily assemblyGenome component analysis: gene structure prediction, repetitive sequences, non-coding RNAsGene function annotation Bacteria Complete MapStandard AnalysisData quality controlGenome assemblyGenome component analysis Repeat annotation
 Coding gene annotation
 ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
COG  annotation
NR annotation,
TCDB annotation
Pfam annotation
Swiss-Prot annotation
Genome cycle graph
Fungus Fine MapStandard AnalysisData quality controlGenome advanced assemblyGenome surveyGenome component analysisRepeat annotation
Coding gene annotation
ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
NR annotation,
KOG annotation
Pfam annotation
Swiss-Prot annotation.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Sequel II/IIe System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb(CCS)
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes ≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft MapStandard AnalysisData quality controlGenome preliminarily assemblyGenome component analysis: gene structure prediction, repetitive sequences, non-coding RNAsGene function annotation Bacteria Complete MapStandard AnalysisData quality controlGenome assemblyGenome component analysis Repeat annotation
 Coding gene annotation
 ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
COG  annotation
NR annotation,
TCDB annotation
Pfam annotation
Swiss-Prot annotation
Genome cycle graph
Fungus Fine MapStandard AnalysisData quality controlGenome advanced assemblyGenome surveyGenome component analysisRepeat annotation
Coding gene annotation
ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
NR annotation,
KOG annotation
Pfam annotation
Swiss-Prot annotation.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Sequel II/IIe System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb(CCS)
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes ≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft MapStandard AnalysisData quality controlGenome preliminarily assemblyGenome component analysis: gene structure prediction, repetitive sequences, non-coding RNAsGene function annotation Bacteria Complete MapStandard AnalysisData quality controlGenome assemblyGenome component analysis Repeat annotation
 Coding gene annotation
 ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
COG  annotation
NR annotation,
TCDB annotation
Pfam annotation
Swiss-Prot annotation
Genome cycle graph
Fungus Fine MapStandard AnalysisData quality controlGenome advanced assemblyGenome surveyGenome component analysisRepeat annotation
Coding gene annotation
ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
NR annotation,
KOG annotation
Pfam annotation
Swiss-Prot annotation.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Sequel II/IIe System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb(CCS)
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes ≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft MapStandard AnalysisData quality controlGenome preliminarily assemblyGenome component analysis: gene structure prediction, repetitive sequences, non-coding RNAsGene function annotation Bacteria Complete MapStandard AnalysisData quality controlGenome assemblyGenome component analysis Repeat annotation
 Coding gene annotation
 ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
COG  annotation
NR annotation,
TCDB annotation
Pfam annotation
Swiss-Prot annotation
Genome cycle graph
Fungus Fine MapStandard AnalysisData quality controlGenome advanced assemblyGenome surveyGenome component analysisRepeat annotation
Coding gene annotation
ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
NR annotation,
KOG annotation
Pfam annotation
Swiss-Prot annotation.

Resources

Example Analysis Output

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Bacterial Genome Map

Visualise assembled bacterial genome features, including coding regions, RNA genes, GC content and GC skew.

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Fungal Genome Map

Review assembled fungal genome features and annotated regions in a circular genome overview.

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GO Functional Annotation

Summarise predicted gene functions by Gene Ontology category to support interpretation of assembled genomes.

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Bacterial Genome Map

Visualise assembled bacterial genome features, including coding regions, RNA genes, GC content and GC skew.

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Fungal Genome Map

Review assembled fungal genome features and annotated regions in a circular genome overview.

Image
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GO Functional Annotation

Summarise predicted gene functions by Gene Ontology category to support interpretation of assembled genomes.

Image
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Bacterial Genome Map

Visualise assembled bacterial genome features, including coding regions, RNA genes, GC content and GC skew.

Image
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Fungal Genome Map

Review assembled fungal genome features and annotated regions in a circular genome overview.

Image
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GO Functional Annotation

Summarise predicted gene functions by Gene Ontology category to support interpretation of assembled genomes.

Webinars

Exploring the Microbial World: A Journey through Sequencing Applications

Explore the latest microbial sequencing approaches, including microbial whole genome sequencing, metagenomics, and metatranscriptomics, and learn how advances such as long-read sequencing are expanding our understanding of microbial communities and ecosystems.

Learn More
(Exploring the Microbial World: A Journey through Sequencing Applications)
Exploring the Microbial World: A Journey through Sequencing Applications

Explore the latest microbial sequencing approaches, including microbial whole genome sequencing, metagenomics, and metatranscriptomics, and learn how advances such as long-read sequencing are expanding our understanding of microbial communities and ecosystems.

Learn More
(Exploring the Microbial World: A Journey through Sequencing Applications)
Exploring the Microbial World: A Journey through Sequencing Applications

Explore the latest microbial sequencing approaches, including microbial whole genome sequencing, metagenomics, and metatranscriptomics, and learn how advances such as long-read sequencing are expanding our understanding of microbial communities and ecosystems.

Learn More
(Exploring the Microbial World: A Journey through Sequencing Applications)
Exploring the Microbial World: A Journey through Sequencing Applications

Explore the latest microbial sequencing approaches, including microbial whole genome sequencing, metagenomics, and metatranscriptomics, and learn how advances such as long-read sequencing are expanding our understanding of microbial communities and ecosystems.

Learn More
(Exploring the Microbial World: A Journey through Sequencing Applications)

Explore Genomics Insights

Access webinars, brochures and case studies covering sequencing technologies, study planning and data interpretation.
View Webinar Library
(Explore Genomics Insights)
View Webinar Library
(Explore Genomics Insights)
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Microbial De novo Sequencing

Genome assembly and reference genome construction for novel, less-characterised and complex microbial genomes.
OverviewOverview
BenefitsBenefits
ApplicationsApplications
SpecificationsSpecifications
ResourcesResources

Microbial de novo sequencing enables genome assembly without relying on an existing reference genome. It is suitable for novel or less-characterised microorganisms, helping researchers construct draft or high-contiguity genomes and explore genome structure, gene content and functional potential.


Novogene Europe supports microbial de novo sequencing projects with short-read and long-read sequencing strategies, quality control and bioinformatics workflows. The service helps researchers generate reliable microbial genome assemblies for comparative genomics, evolutionary studies and applied microbiology research.

Service Highlights

Reference-free Assembly
Reference-free Assembly

Assemble microbial genomes without an existing reference, supporting genome construction for novel, less-characterised or newly isolated microorganisms.

Reference-free Assembly
Reference-free Assembly

Assemble microbial genomes without an existing reference, supporting genome construction for novel, less-characterised or newly isolated microorganisms.

Flexible Sequencing Strategies
Flexible Sequencing Strategies

Short- or long-read sequencing can be selected based on microbial genome size, sample quality and assembly goals, supporting draft assemblies or higher-contiguity genome reconstruction.

Flexible Sequencing Strategies
Flexible Sequencing Strategies

Short- or long-read sequencing can be selected based on microbial genome size, sample quality and assembly goals, supporting draft assemblies or higher-contiguity genome reconstruction.

Assembly Quality Assessment
Assembly Quality Assessment

Evaluate genome assembly outputs using key metrics such as coverage, contiguity and completeness to support reliable downstream analysis.

Assembly Quality Assessment
Assembly Quality Assessment

Evaluate genome assembly outputs using key metrics such as coverage, contiguity and completeness to support reliable downstream analysis.

Genome Annotation Support
Genome Annotation Support

Bioinformatics workflows can support gene prediction, functional annotation and comparative analysis to help characterise microbial genome resources.

Genome Annotation Support
Genome Annotation Support

Bioinformatics workflows can support gene prediction, functional annotation and comparative analysis to help characterise microbial genome resources.

Service Highlights

Reference-free Assembly
Reference-free Assembly

Assemble microbial genomes without an existing reference, supporting genome construction for novel, less-characterised or newly isolated microorganisms.

Reference-free Assembly
Reference-free Assembly

Assemble microbial genomes without an existing reference, supporting genome construction for novel, less-characterised or newly isolated microorganisms.

Flexible Sequencing Strategies
Flexible Sequencing Strategies

Short- or long-read sequencing can be selected based on microbial genome size, sample quality and assembly goals, supporting draft assemblies or higher-contiguity genome reconstruction.

Flexible Sequencing Strategies
Flexible Sequencing Strategies

Short- or long-read sequencing can be selected based on microbial genome size, sample quality and assembly goals, supporting draft assemblies or higher-contiguity genome reconstruction.

Assembly Quality Assessment
Assembly Quality Assessment

Evaluate genome assembly outputs using key metrics such as coverage, contiguity and completeness to support reliable downstream analysis.

Assembly Quality Assessment
Assembly Quality Assessment

Evaluate genome assembly outputs using key metrics such as coverage, contiguity and completeness to support reliable downstream analysis.

Genome Annotation Support
Genome Annotation Support

Bioinformatics workflows can support gene prediction, functional annotation and comparative analysis to help characterise microbial genome resources.

Genome Annotation Support
Genome Annotation Support

Bioinformatics workflows can support gene prediction, functional annotation and comparative analysis to help characterise microbial genome resources.

Applications

Novel Microorganism Research

Microbial de novo sequencing supports genome analysis for novel or less-characterised microorganisms where a suitable reference genome may not be available.

Novel Microorganism Research

Microbial de novo sequencing supports genome analysis for novel or less-characterised microorganisms where a suitable reference genome may not be available.

Genome Resource Development

De novo assembly can help researchers generate draft or high-contiguity genome resources for downstream annotation, comparison and future genomic studies.

Genome Resource Development

De novo assembly can help researchers generate draft or high-contiguity genome resources for downstream annotation, comparison and future genomic studies.

Functional Genome Exploration

Assembled microbial genomes support gene prediction and functional annotation, helping researchers explore metabolism, adaptation and biological potential.

Functional Genome Exploration

Assembled microbial genomes support gene prediction and functional annotation, helping researchers explore metabolism, adaptation and biological potential.

Evolutionary and Ecological Studies

Microbial genome assemblies can support studies of genome evolution, microbial diversity and adaptation across environmental, agricultural or ecological contexts.

Evolutionary and Ecological Studies

Microbial genome assemblies can support studies of genome evolution, microbial diversity and adaptation across environmental, agricultural or ecological contexts.

Applications

Novel Microorganism Research

Microbial de novo sequencing supports genome analysis for novel or less-characterised microorganisms where a suitable reference genome may not be available.

Novel Microorganism Research

Microbial de novo sequencing supports genome analysis for novel or less-characterised microorganisms where a suitable reference genome may not be available.

Genome Resource Development

De novo assembly can help researchers generate draft or high-contiguity genome resources for downstream annotation, comparison and future genomic studies.

Genome Resource Development

De novo assembly can help researchers generate draft or high-contiguity genome resources for downstream annotation, comparison and future genomic studies.

Functional Genome Exploration

Assembled microbial genomes support gene prediction and functional annotation, helping researchers explore metabolism, adaptation and biological potential.

Functional Genome Exploration

Assembled microbial genomes support gene prediction and functional annotation, helping researchers explore metabolism, adaptation and biological potential.

Evolutionary and Ecological Studies

Microbial genome assemblies can support studies of genome evolution, microbial diversity and adaptation across environmental, agricultural or ecological contexts.

Evolutionary and Ecological Studies

Microbial genome assemblies can support studies of genome evolution, microbial diversity and adaptation across environmental, agricultural or ecological contexts.

Specifications

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

ServiceSample TypeAmount (Qubit®)VolumeConcentrationPurity (NanoDrop™/Agarose Gel)
Microbial de novo sequencingGenomic DNA/ total DNA*≥100 ng≥20 μL≥5 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination Fragments
longer than 1000 bp
PCR-free WGSGenomic DNA / total DNA≥1.2 μg≥20 μL≥20 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination
*Total DNA includes all types of DNA present in a sample, including genomic DNA, mitochondrial DNA and any other extrachromosomal DNA.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

ServiceSample TypeAmount (Qubit®)VolumeConcentrationPurity (NanoDrop™/Agarose Gel)
Microbial de novo sequencingGenomic DNA/ total DNA*≥100 ng≥20 μL≥5 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination Fragments
longer than 1000 bp
PCR-free WGSGenomic DNA / total DNA≥1.2 μg≥20 μL≥20 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination
*Total DNA includes all types of DNA present in a sample, including genomic DNA, mitochondrial DNA and any other extrachromosomal DNA.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

ServiceSample TypeAmount (Qubit®)VolumeConcentrationPurity (NanoDrop™/Agarose Gel)
Microbial de novo sequencingGenomic DNA/ total DNA*≥100 ng≥20 μL≥5 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination Fragments
longer than 1000 bp
PCR-free WGSGenomic DNA / total DNA≥1.2 μg≥20 μL≥20 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination
*Total DNA includes all types of DNA present in a sample, including genomic DNA, mitochondrial DNA and any other extrachromosomal DNA.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

ServiceSample TypeAmount (Qubit®)VolumeConcentrationPurity (NanoDrop™/Agarose Gel)
Microbial de novo sequencingGenomic DNA/ total DNA*≥100 ng≥20 μL≥5 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination Fragments
longer than 1000 bp
PCR-free WGSGenomic DNA / total DNA≥1.2 μg≥20 μL≥20 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination
*Total DNA includes all types of DNA present in a sample, including genomic DNA, mitochondrial DNA and any other extrachromosomal DNA.
erviceSample TypeAmount (Qubit®)VolumeConcentrationPurity
PacBio DNA HiFi libraryHMW genomic DNA (Bacteria & Fungus)≥1.5 μg≥30 μL≥50 ng/μLOD260/280=1.75~2.2;
OD260/230=1.3~2.6;
NC/QC=1.00~2.20
Fragments should be ≥20K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
erviceSample TypeAmount (Qubit®)VolumeConcentrationPurity
PacBio DNA HiFi libraryHMW genomic DNA (Bacteria & Fungus)≥1.5 μg≥30 μL≥50 ng/μLOD260/280=1.75~2.2;
OD260/230=1.3~2.6;
NC/QC=1.00~2.20
Fragments should be ≥20K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
erviceSample TypeAmount (Qubit®)VolumeConcentrationPurity
PacBio DNA HiFi libraryHMW genomic DNA (Bacteria & Fungus)≥1.5 μg≥30 μL≥50 ng/μLOD260/280=1.75~2.2;
OD260/230=1.3~2.6;
NC/QC=1.00~2.20
Fragments should be ≥20K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
erviceSample TypeAmount (Qubit®)VolumeConcentrationPurity
PacBio DNA HiFi libraryHMW genomic DNA (Bacteria & Fungus)≥1.5 μg≥30 μL≥50 ng/μLOD260/280=1.75~2.2;
OD260/230=1.3~2.6;
NC/QC=1.00~2.20
Fragments should be ≥20K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
ServiceSample TypeRecommended Amount VolumeConcentrationPurity
Nanopore PromethION DNA libraryHMW genomic DNA (Bacteria & Fungus)≥3 μg≥50 μL≥60 ng/μLOD260/280=1.7~2.2;
OD260/230=1.3~2.6;
NC/QC=0.95~3.00
Fragments should be ≥15K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
ServiceSample TypeRecommended Amount VolumeConcentrationPurity
Nanopore PromethION DNA libraryHMW genomic DNA (Bacteria & Fungus)≥3 μg≥50 μL≥60 ng/μLOD260/280=1.7~2.2;
OD260/230=1.3~2.6;
NC/QC=0.95~3.00
Fragments should be ≥15K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
ServiceSample TypeRecommended Amount VolumeConcentrationPurity
Nanopore PromethION DNA libraryHMW genomic DNA (Bacteria & Fungus)≥3 μg≥50 μL≥60 ng/μLOD260/280=1.7~2.2;
OD260/230=1.3~2.6;
NC/QC=0.95~3.00
Fragments should be ≥15K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
ServiceSample TypeRecommended Amount VolumeConcentrationPurity
Nanopore PromethION DNA libraryHMW genomic DNA (Bacteria & Fungus)≥3 μg≥50 μL≥60 ng/μLOD260/280=1.7~2.2;
OD260/230=1.3~2.6;
NC/QC=0.95~3.00
Fragments should be ≥15K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Sequel II/IIe System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb(CCS)
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes ≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft MapStandard AnalysisData quality controlGenome preliminarily assemblyGenome component analysis: gene structure prediction, repetitive sequences, non-coding RNAsGene function annotation Bacteria Complete MapStandard AnalysisData quality controlGenome assemblyGenome component analysis Repeat annotation
 Coding gene annotation
 ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
COG  annotation
NR annotation,
TCDB annotation
Pfam annotation
Swiss-Prot annotation
Genome cycle graph
Fungus Fine MapStandard AnalysisData quality controlGenome advanced assemblyGenome surveyGenome component analysisRepeat annotation
Coding gene annotation
ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
NR annotation,
KOG annotation
Pfam annotation
Swiss-Prot annotation.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Sequel II/IIe System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb(CCS)
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes ≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft MapStandard AnalysisData quality controlGenome preliminarily assemblyGenome component analysis: gene structure prediction, repetitive sequences, non-coding RNAsGene function annotation Bacteria Complete MapStandard AnalysisData quality controlGenome assemblyGenome component analysis Repeat annotation
 Coding gene annotation
 ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
COG  annotation
NR annotation,
TCDB annotation
Pfam annotation
Swiss-Prot annotation
Genome cycle graph
Fungus Fine MapStandard AnalysisData quality controlGenome advanced assemblyGenome surveyGenome component analysisRepeat annotation
Coding gene annotation
ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
NR annotation,
KOG annotation
Pfam annotation
Swiss-Prot annotation.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Sequel II/IIe System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb(CCS)
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes ≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft MapStandard AnalysisData quality controlGenome preliminarily assemblyGenome component analysis: gene structure prediction, repetitive sequences, non-coding RNAsGene function annotation Bacteria Complete MapStandard AnalysisData quality controlGenome assemblyGenome component analysis Repeat annotation
 Coding gene annotation
 ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
COG  annotation
NR annotation,
TCDB annotation
Pfam annotation
Swiss-Prot annotation
Genome cycle graph
Fungus Fine MapStandard AnalysisData quality controlGenome advanced assemblyGenome surveyGenome component analysisRepeat annotation
Coding gene annotation
ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
NR annotation,
KOG annotation
Pfam annotation
Swiss-Prot annotation.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Sequel II/IIe System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb(CCS)
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes ≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft MapStandard AnalysisData quality controlGenome preliminarily assemblyGenome component analysis: gene structure prediction, repetitive sequences, non-coding RNAsGene function annotation Bacteria Complete MapStandard AnalysisData quality controlGenome assemblyGenome component analysis Repeat annotation
 Coding gene annotation
 ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
COG  annotation
NR annotation,
TCDB annotation
Pfam annotation
Swiss-Prot annotation
Genome cycle graph
Fungus Fine MapStandard AnalysisData quality controlGenome advanced assemblyGenome surveyGenome component analysisRepeat annotation
Coding gene annotation
ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
NR annotation,
KOG annotation
Pfam annotation
Swiss-Prot annotation.

Specifications

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

ServiceSample TypeAmount (Qubit®)VolumeConcentrationPurity (NanoDrop™/Agarose Gel)
Microbial de novo sequencingGenomic DNA/ total DNA*≥100 ng≥20 μL≥5 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination Fragments
longer than 1000 bp
PCR-free WGSGenomic DNA / total DNA≥1.2 μg≥20 μL≥20 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination
*Total DNA includes all types of DNA present in a sample, including genomic DNA, mitochondrial DNA and any other extrachromosomal DNA.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

ServiceSample TypeAmount (Qubit®)VolumeConcentrationPurity (NanoDrop™/Agarose Gel)
Microbial de novo sequencingGenomic DNA/ total DNA*≥100 ng≥20 μL≥5 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination Fragments
longer than 1000 bp
PCR-free WGSGenomic DNA / total DNA≥1.2 μg≥20 μL≥20 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination
*Total DNA includes all types of DNA present in a sample, including genomic DNA, mitochondrial DNA and any other extrachromosomal DNA.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

ServiceSample TypeAmount (Qubit®)VolumeConcentrationPurity (NanoDrop™/Agarose Gel)
Microbial de novo sequencingGenomic DNA/ total DNA*≥100 ng≥20 μL≥5 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination Fragments
longer than 1000 bp
PCR-free WGSGenomic DNA / total DNA≥1.2 μg≥20 μL≥20 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination
*Total DNA includes all types of DNA present in a sample, including genomic DNA, mitochondrial DNA and any other extrachromosomal DNA.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

ServiceSample TypeAmount (Qubit®)VolumeConcentrationPurity (NanoDrop™/Agarose Gel)
Microbial de novo sequencingGenomic DNA/ total DNA*≥100 ng≥20 μL≥5 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination Fragments
longer than 1000 bp
PCR-free WGSGenomic DNA / total DNA≥1.2 μg≥20 μL≥20 ng/μLOD260/280 = 1.8-2.0;
no degradation, no
contamination
*Total DNA includes all types of DNA present in a sample, including genomic DNA, mitochondrial DNA and any other extrachromosomal DNA.
erviceSample TypeAmount (Qubit®)VolumeConcentrationPurity
PacBio DNA HiFi libraryHMW genomic DNA (Bacteria & Fungus)≥1.5 μg≥30 μL≥50 ng/μLOD260/280=1.75~2.2;
OD260/230=1.3~2.6;
NC/QC=1.00~2.20
Fragments should be ≥20K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
erviceSample TypeAmount (Qubit®)VolumeConcentrationPurity
PacBio DNA HiFi libraryHMW genomic DNA (Bacteria & Fungus)≥1.5 μg≥30 μL≥50 ng/μLOD260/280=1.75~2.2;
OD260/230=1.3~2.6;
NC/QC=1.00~2.20
Fragments should be ≥20K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
erviceSample TypeAmount (Qubit®)VolumeConcentrationPurity
PacBio DNA HiFi libraryHMW genomic DNA (Bacteria & Fungus)≥1.5 μg≥30 μL≥50 ng/μLOD260/280=1.75~2.2;
OD260/230=1.3~2.6;
NC/QC=1.00~2.20
Fragments should be ≥20K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
erviceSample TypeAmount (Qubit®)VolumeConcentrationPurity
PacBio DNA HiFi libraryHMW genomic DNA (Bacteria & Fungus)≥1.5 μg≥30 μL≥50 ng/μLOD260/280=1.75~2.2;
OD260/230=1.3~2.6;
NC/QC=1.00~2.20
Fragments should be ≥20K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
ServiceSample TypeRecommended Amount VolumeConcentrationPurity
Nanopore PromethION DNA libraryHMW genomic DNA (Bacteria & Fungus)≥3 μg≥50 μL≥60 ng/μLOD260/280=1.7~2.2;
OD260/230=1.3~2.6;
NC/QC=0.95~3.00
Fragments should be ≥15K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
ServiceSample TypeRecommended Amount VolumeConcentrationPurity
Nanopore PromethION DNA libraryHMW genomic DNA (Bacteria & Fungus)≥3 μg≥50 μL≥60 ng/μLOD260/280=1.7~2.2;
OD260/230=1.3~2.6;
NC/QC=0.95~3.00
Fragments should be ≥15K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
ServiceSample TypeRecommended Amount VolumeConcentrationPurity
Nanopore PromethION DNA libraryHMW genomic DNA (Bacteria & Fungus)≥3 μg≥50 μL≥60 ng/μLOD260/280=1.7~2.2;
OD260/230=1.3~2.6;
NC/QC=0.95~3.00
Fragments should be ≥15K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration
ServiceSample TypeRecommended Amount VolumeConcentrationPurity
Nanopore PromethION DNA libraryHMW genomic DNA (Bacteria & Fungus)≥3 μg≥50 μL≥60 ng/μLOD260/280=1.7~2.2;
OD260/230=1.3~2.6;
NC/QC=0.95~3.00
Fragments should be ≥15K
*HMW = High Molecular Weight; NC/QC = NanoDrop concentration/Qubit concentration

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Sequel II/IIe System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb(CCS)
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes ≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft MapStandard AnalysisData quality controlGenome preliminarily assemblyGenome component analysis: gene structure prediction, repetitive sequences, non-coding RNAsGene function annotation Bacteria Complete MapStandard AnalysisData quality controlGenome assemblyGenome component analysis Repeat annotation
 Coding gene annotation
 ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
COG  annotation
NR annotation,
TCDB annotation
Pfam annotation
Swiss-Prot annotation
Genome cycle graph
Fungus Fine MapStandard AnalysisData quality controlGenome advanced assemblyGenome surveyGenome component analysisRepeat annotation
Coding gene annotation
ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
NR annotation,
KOG annotation
Pfam annotation
Swiss-Prot annotation.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Sequel II/IIe System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb(CCS)
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes ≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft MapStandard AnalysisData quality controlGenome preliminarily assemblyGenome component analysis: gene structure prediction, repetitive sequences, non-coding RNAsGene function annotation Bacteria Complete MapStandard AnalysisData quality controlGenome assemblyGenome component analysis Repeat annotation
 Coding gene annotation
 ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
COG  annotation
NR annotation,
TCDB annotation
Pfam annotation
Swiss-Prot annotation
Genome cycle graph
Fungus Fine MapStandard AnalysisData quality controlGenome advanced assemblyGenome surveyGenome component analysisRepeat annotation
Coding gene annotation
ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
NR annotation,
KOG annotation
Pfam annotation
Swiss-Prot annotation.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Sequel II/IIe System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb(CCS)
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes ≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft MapStandard AnalysisData quality controlGenome preliminarily assemblyGenome component analysis: gene structure prediction, repetitive sequences, non-coding RNAsGene function annotation Bacteria Complete MapStandard AnalysisData quality controlGenome assemblyGenome component analysis Repeat annotation
 Coding gene annotation
 ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
COG  annotation
NR annotation,
TCDB annotation
Pfam annotation
Swiss-Prot annotation
Genome cycle graph
Fungus Fine MapStandard AnalysisData quality controlGenome advanced assemblyGenome surveyGenome component analysisRepeat annotation
Coding gene annotation
ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
NR annotation,
KOG annotation
Pfam annotation
Swiss-Prot annotation.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Platform TypeIllumina NovaSeqPacBio Sequel II/IIe System
Read LengthPaired-end 150 bpN50>15 kb, long read lengths up to 25 kb(CCS)
Recommended Sequencing Depth≥ 50x for bacterial and fungal genomes≥ 100X for bacterial genomes ≥ 50X for fungal genomes
Data AnalysisBacterial and Fungal Draft MapStandard AnalysisData quality controlGenome preliminarily assemblyGenome component analysis: gene structure prediction, repetitive sequences, non-coding RNAsGene function annotation Bacteria Complete MapStandard AnalysisData quality controlGenome assemblyGenome component analysis Repeat annotation
 Coding gene annotation
 ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
COG  annotation
NR annotation,
TCDB annotation
Pfam annotation
Swiss-Prot annotation
Genome cycle graph
Fungus Fine MapStandard AnalysisData quality controlGenome advanced assemblyGenome surveyGenome component analysisRepeat annotation
Coding gene annotation
ncRNA annotation
Gene function annotationGO annotation,
KEGG annotation,
NR annotation,
KOG annotation
Pfam annotation
Swiss-Prot annotation.

Resources

Example Analysis Output

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Bacterial Genome Map

Visualise assembled bacterial genome features, including coding regions, RNA genes, GC content and GC skew.

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Fungal Genome Map

Review assembled fungal genome features and annotated regions in a circular genome overview.

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GO Functional Annotation

Summarise predicted gene functions by Gene Ontology category to support interpretation of assembled genomes.

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Bacterial Genome Map

Visualise assembled bacterial genome features, including coding regions, RNA genes, GC content and GC skew.

Image
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Fungal Genome Map

Review assembled fungal genome features and annotated regions in a circular genome overview.

Image
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1/1
GO Functional Annotation

Summarise predicted gene functions by Gene Ontology category to support interpretation of assembled genomes.

Image
Image
1/1
Bacterial Genome Map

Visualise assembled bacterial genome features, including coding regions, RNA genes, GC content and GC skew.

Image
Image
1/1
Fungal Genome Map

Review assembled fungal genome features and annotated regions in a circular genome overview.

Image
Image
1/1
GO Functional Annotation

Summarise predicted gene functions by Gene Ontology category to support interpretation of assembled genomes.

Webinars

Exploring the Microbial World: A Journey through Sequencing Applications

Explore the latest microbial sequencing approaches, including microbial whole genome sequencing, metagenomics, and metatranscriptomics, and learn how advances such as long-read sequencing are expanding our understanding of microbial communities and ecosystems.

Learn More
(Exploring the Microbial World: A Journey through Sequencing Applications)
Exploring the Microbial World: A Journey through Sequencing Applications

Explore the latest microbial sequencing approaches, including microbial whole genome sequencing, metagenomics, and metatranscriptomics, and learn how advances such as long-read sequencing are expanding our understanding of microbial communities and ecosystems.

Learn More
(Exploring the Microbial World: A Journey through Sequencing Applications)
Exploring the Microbial World: A Journey through Sequencing Applications

Explore the latest microbial sequencing approaches, including microbial whole genome sequencing, metagenomics, and metatranscriptomics, and learn how advances such as long-read sequencing are expanding our understanding of microbial communities and ecosystems.

Learn More
(Exploring the Microbial World: A Journey through Sequencing Applications)
Exploring the Microbial World: A Journey through Sequencing Applications

Explore the latest microbial sequencing approaches, including microbial whole genome sequencing, metagenomics, and metatranscriptomics, and learn how advances such as long-read sequencing are expanding our understanding of microbial communities and ecosystems.

Learn More
(Exploring the Microbial World: A Journey through Sequencing Applications)

Explore Genomics Insights

Access webinars, brochures and case studies covering sequencing technologies, study planning and data interpretation.
View Webinar Library
(Explore Genomics Insights)
View Webinar Library
(Explore Genomics Insights)
Background
Background

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