• Cart
    • Quote
    • Enquiry
    • Cart
    • Quote
    • Enquiry
Novogene Europe
  • Novogene Europe
  • Genomics
    • Human Whole Genome Sequencing
    • Whole Exome Sequencing
    • Plant and Animal Whole Genome Sequencing
    • Plant and Animal De novo Sequencing
    • Microbial Whole Genome Sequencing
    • Microbial De novo Sequencing
    • Whole Plasmid SequencingOrder Online!
    Proteomics
    • Olink ProteomicsNew!
    Epigenomics
    • DNA Methylation SequencingUpdated!
    • Chromatin Immunoprecipitation Sequencing (ChIP-seq)
    • RNA Immunoprecipitation Sequencing (RIP-seq)
    Metabolomics
    • Untargeted MetabolomicsComing Soon!
    Transcriptomics
    • mRNA Sequencing
    • Small RNA Sequencing (sRNA‑seq)
    • Circular RNA Sequencing (circRNA-seq)
    • Total RNA Sequencing
    • Whole Transcriptome Sequencing
    • Full-Length Transcriptome Sequencing
    • Prokaryotic RNA Sequencing
    • Metatranscriptome Sequencing
    Metagenomics
    • Amplicon SequencingOrder Online!
    • Shotgun Metagenomics Sequencing
    Single Cell & Spatial Omics
    • 10X Single Cell Gene Expression
    • 10X Single Cell Immune Profiling
    • 10X Visium HD Spatial Gene Expression
    Premade Library
    • Sequencing Only on Illumina® Sequencer
    • Sequencing Only on Element SequencerNew!
    • Sequencing Only on PacBio Sequencer
    Translational Research
    • Accredited & Validated Clinical Research Sequencing
  • PromotionsPromotions
    • Platforms
    • Automated Delivery Platform (Falcon)
    • Bioinformatics Analysis Tool (NovoMagic)
    • Customer Service System (CSS)
    • Customer Support
    • Webinars
    • Case Study
    • Blog
    • Brochure
    • Cancer Research
    • Immuno-oncology
    • Agrigenomics
    • Environment
    • Food Science
    • Human Microbiome
    • Plant and Animal Microbiome
    • Drug Discovery and Development
    • Rare and Complex Diseases
    • About Us
    • News & Events
    • Careers
    • Our Locations
  • Contact UsContact Us
    • Amplicon Sequencing
    • Whole Plasmid Sequencing

ServicesServices menu

ResourcesResources menu

SupportSupport menu

CompanyCompany menu

Services
Human Whole Genome SequencingWhole Exome SequencingPlant and Animal Whole Genome SequencingPlant and Animal De novo SequencingDNA Methylation SequencingmRNA SequencingFull-Length Transcriptome SequencingWhole Transcriptome SequencingMetatranscriptome SequencingShotgun Metagenomics SequencingAmplicon SequencingWhole Plasmid Sequencing10X Single Cell Gene Expression10X Single Cell Immune Profiling10X Visium HD Spatial Gene ExpressionOlink ProteomicsUntargeted MetabolomicsAccredited & Validated Clinical Research Sequencing
Resources
WebinarsCase StudyBlogBrochure
Support
PlatformBioinformatics Analysis Tool (NovoMagic)Customer Service System (CSS)Customer Support
Company
About UsNews & EventsLocationContact Us
LinkedInLinkedIn hoverYouTubeYouTube hoverInstagramInstagram hoverXX hover
Copyright © 2026 Novogene Inc. All rights reserved.For Research Use Only. Not for Clinical Diagnostic Use.
    • Cart
    • Quote
    • Enquiry
    • Cart
    • Quote
    • Enquiry
Novogene Europe
  • Novogene Europe
  • Genomics
    • Human Whole Genome Sequencing
    • Whole Exome Sequencing
    • Plant and Animal Whole Genome Sequencing
    • Plant and Animal De novo Sequencing
    • Microbial Whole Genome Sequencing
    • Microbial De novo Sequencing
    • Whole Plasmid SequencingOrder Online!
    Proteomics
    • Olink ProteomicsNew!
    Epigenomics
    • DNA Methylation SequencingUpdated!
    • Chromatin Immunoprecipitation Sequencing (ChIP-seq)
    • RNA Immunoprecipitation Sequencing (RIP-seq)
    Metabolomics
    • Untargeted MetabolomicsComing Soon!
    Transcriptomics
    • mRNA Sequencing
    • Small RNA Sequencing (sRNA‑seq)
    • Circular RNA Sequencing (circRNA-seq)
    • Total RNA Sequencing
    • Whole Transcriptome Sequencing
    • Full-Length Transcriptome Sequencing
    • Prokaryotic RNA Sequencing
    • Metatranscriptome Sequencing
    Metagenomics
    • Amplicon SequencingOrder Online!
    • Shotgun Metagenomics Sequencing
    Single Cell & Spatial Omics
    • 10X Single Cell Gene Expression
    • 10X Single Cell Immune Profiling
    • 10X Visium HD Spatial Gene Expression
    Premade Library
    • Sequencing Only on Illumina® Sequencer
    • Sequencing Only on Element SequencerNew!
    • Sequencing Only on PacBio Sequencer
    Translational Research
    • Accredited & Validated Clinical Research Sequencing
  • PromotionsPromotions
    • Platforms
    • Automated Delivery Platform (Falcon)
    • Bioinformatics Analysis Tool (NovoMagic)
    • Customer Service System (CSS)
    • Customer Support
    • Webinars
    • Case Study
    • Blog
    • Brochure
    • Cancer Research
    • Immuno-oncology
    • Agrigenomics
    • Environment
    • Food Science
    • Human Microbiome
    • Plant and Animal Microbiome
    • Drug Discovery and Development
    • Rare and Complex Diseases
    • About Us
    • News & Events
    • Careers
    • Our Locations
  • Contact UsContact Us
    • Amplicon Sequencing
    • Whole Plasmid Sequencing

ServicesServices menu

ResourcesResources menu

SupportSupport menu

CompanyCompany menu

Services
Human Whole Genome SequencingWhole Exome SequencingPlant and Animal Whole Genome SequencingPlant and Animal De novo SequencingDNA Methylation SequencingmRNA SequencingFull-Length Transcriptome SequencingWhole Transcriptome SequencingMetatranscriptome SequencingShotgun Metagenomics SequencingAmplicon SequencingWhole Plasmid Sequencing10X Single Cell Gene Expression10X Single Cell Immune Profiling10X Visium HD Spatial Gene ExpressionOlink ProteomicsUntargeted MetabolomicsAccredited & Validated Clinical Research Sequencing
Resources
WebinarsCase StudyBlogBrochure
Support
PlatformBioinformatics Analysis Tool (NovoMagic)Customer Service System (CSS)Customer Support
Company
About UsNews & EventsLocationContact Us
LinkedInLinkedIn hoverYouTubeYouTube hoverInstagramInstagram hoverXX hover
Copyright © 2026 Novogene Inc. All rights reserved.For Research Use Only. Not for Clinical Diagnostic Use.
banner laptop bg
banner mobile bg

Metatranscriptome Sequencing

Community-wide RNA sequencing to explore active microbial gene expression, functional activity and environmental response.
OverviewOverview
BenefitsBenefits
ApplicationsApplications
SpecificationsSpecifications
ResourcesResources

Metatranscriptome sequencing enables transcriptome-wide analysis of actively expressed genes within complex microbial communities. Unlike DNA-based metagenomics, which profiles genetic potential, metatranscriptomics helps reveal which microbial genes and pathways are active under specific environmental, host-associated or experimental conditions.


Novogene Europe supports metatranscriptome sequencing projects using high-throughput RNA sequencing and bioinformatics workflows for metatranscriptome assembly, species annotation, functional annotation, taxonomic analysis, gene expression quantification and differential expression analysis. This approach can help researchers investigate microbial community function, pathway activity and adaptive responses across complex ecosystems.

Service Highlights

Active Community Expression Profiling
Active Community Expression Profiling

Profile expressed microbial genes across complex communities to investigate functional activity under specific host, environmental or experimental conditions.

Active Community Expression Profiling
Active Community Expression Profiling

Profile expressed microbial genes across complex communities to investigate functional activity under specific host, environmental or experimental conditions.

Taxonomic and Functional Annotation
Taxonomic and Functional Annotation

Bioinformatics workflows support species annotation, functional annotation and pathway-level interpretation of expressed microbial transcripts.

Taxonomic and Functional Annotation
Taxonomic and Functional Annotation

Bioinformatics workflows support species annotation, functional annotation and pathway-level interpretation of expressed microbial transcripts.

Metatranscriptome Assembly Support
Metatranscriptome Assembly Support

Assembly workflows help reconstruct expressed gene sequences from complex microbial RNA data, supporting downstream annotation and expression analysis.

Metatranscriptome Assembly Support
Metatranscriptome Assembly Support

Assembly workflows help reconstruct expressed gene sequences from complex microbial RNA data, supporting downstream annotation and expression analysis.

Differential Expression Analysis
Differential Expression Analysis

Compare microbial gene expression across sample groups to explore community-level functional changes, pathway activity and adaptive responses.

Differential Expression Analysis
Differential Expression Analysis

Compare microbial gene expression across sample groups to explore community-level functional changes, pathway activity and adaptive responses.

Service Highlights

Active Community Expression Profiling
Active Community Expression Profiling

Profile expressed microbial genes across complex communities to investigate functional activity under specific host, environmental or experimental conditions.

Active Community Expression Profiling
Active Community Expression Profiling

Profile expressed microbial genes across complex communities to investigate functional activity under specific host, environmental or experimental conditions.

Taxonomic and Functional Annotation
Taxonomic and Functional Annotation

Bioinformatics workflows support species annotation, functional annotation and pathway-level interpretation of expressed microbial transcripts.

Taxonomic and Functional Annotation
Taxonomic and Functional Annotation

Bioinformatics workflows support species annotation, functional annotation and pathway-level interpretation of expressed microbial transcripts.

Metatranscriptome Assembly Support
Metatranscriptome Assembly Support

Assembly workflows help reconstruct expressed gene sequences from complex microbial RNA data, supporting downstream annotation and expression analysis.

Metatranscriptome Assembly Support
Metatranscriptome Assembly Support

Assembly workflows help reconstruct expressed gene sequences from complex microbial RNA data, supporting downstream annotation and expression analysis.

Differential Expression Analysis
Differential Expression Analysis

Compare microbial gene expression across sample groups to explore community-level functional changes, pathway activity and adaptive responses.

Differential Expression Analysis
Differential Expression Analysis

Compare microbial gene expression across sample groups to explore community-level functional changes, pathway activity and adaptive responses.

Applications

Microbial Community Function

Metatranscriptome sequencing helps researchers investigate which microbial genes and pathways are actively expressed across complex community samples.

Microbial Community Function

Metatranscriptome sequencing helps researchers investigate which microbial genes and pathways are actively expressed across complex community samples.

Host–Microbe Interaction

Community RNA profiles can support studies of microbial activity in host-associated environments, including gut, skin, oral, infection or disease-related models.

Host–Microbe Interaction

Community RNA profiles can support studies of microbial activity in host-associated environments, including gut, skin, oral, infection or disease-related models.

Environmental and Ecological Response

Metatranscriptomics can help explore how microbial communities respond to environmental change, nutrient availability, stress or experimental treatments.

Environmental and Ecological Response

Metatranscriptomics can help explore how microbial communities respond to environmental change, nutrient availability, stress or experimental treatments.

Pathway and Functional Adaptation

Expressed gene and pathway analysis can support research into microbial metabolism, functional adaptation and community-level biological responses.

Pathway and Functional Adaptation

Expressed gene and pathway analysis can support research into microbial metabolism, functional adaptation and community-level biological responses.

Applications

Microbial Community Function

Metatranscriptome sequencing helps researchers investigate which microbial genes and pathways are actively expressed across complex community samples.

Microbial Community Function

Metatranscriptome sequencing helps researchers investigate which microbial genes and pathways are actively expressed across complex community samples.

Host–Microbe Interaction

Community RNA profiles can support studies of microbial activity in host-associated environments, including gut, skin, oral, infection or disease-related models.

Host–Microbe Interaction

Community RNA profiles can support studies of microbial activity in host-associated environments, including gut, skin, oral, infection or disease-related models.

Environmental and Ecological Response

Metatranscriptomics can help explore how microbial communities respond to environmental change, nutrient availability, stress or experimental treatments.

Environmental and Ecological Response

Metatranscriptomics can help explore how microbial communities respond to environmental change, nutrient availability, stress or experimental treatments.

Pathway and Functional Adaptation

Expressed gene and pathway analysis can support research into microbial metabolism, functional adaptation and community-level biological responses.

Pathway and Functional Adaptation

Expressed gene and pathway analysis can support research into microbial metabolism, functional adaptation and community-level biological responses.

Specifications

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity NumberPurity
Meta-transcriptome LibraryTotal RNA≥ 300 ng≥ 5.8, with flat baselineOD260/280≥2.0.
OD260/230≥2.0.
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity NumberPurity
Meta-transcriptome LibraryTotal RNA≥ 300 ng≥ 5.8, with flat baselineOD260/280≥2.0.
OD260/230≥2.0.
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity NumberPurity
Meta-transcriptome LibraryTotal RNA≥ 300 ng≥ 5.8, with flat baselineOD260/280≥2.0.
OD260/230≥2.0.
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity NumberPurity
Meta-transcriptome LibraryTotal RNA≥ 300 ng≥ 5.8, with flat baselineOD260/280≥2.0.
OD260/230≥2.0.
no degradation,
no contamination

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina Sequencing System
Read LengthPaired-end 150bp
Recommended Data Amount≥ 12 Gb per sample
Content of Data AnalysisData Quality Control
Metatranscriptome Assembly
Species Annotation
Functional Annotation
Taxonomic Analysis
Gene expression quantification & Differential expression analysis
Enrichment Analysis

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina Sequencing System
Read LengthPaired-end 150bp
Recommended Data Amount≥ 12 Gb per sample
Content of Data AnalysisData Quality Control
Metatranscriptome Assembly
Species Annotation
Functional Annotation
Taxonomic Analysis
Gene expression quantification & Differential expression analysis
Enrichment Analysis

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina Sequencing System
Read LengthPaired-end 150bp
Recommended Data Amount≥ 12 Gb per sample
Content of Data AnalysisData Quality Control
Metatranscriptome Assembly
Species Annotation
Functional Annotation
Taxonomic Analysis
Gene expression quantification & Differential expression analysis
Enrichment Analysis

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina Sequencing System
Read LengthPaired-end 150bp
Recommended Data Amount≥ 12 Gb per sample
Content of Data AnalysisData Quality Control
Metatranscriptome Assembly
Species Annotation
Functional Annotation
Taxonomic Analysis
Gene expression quantification & Differential expression analysis
Enrichment Analysis

Specifications

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity NumberPurity
Meta-transcriptome LibraryTotal RNA≥ 300 ng≥ 5.8, with flat baselineOD260/280≥2.0.
OD260/230≥2.0.
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity NumberPurity
Meta-transcriptome LibraryTotal RNA≥ 300 ng≥ 5.8, with flat baselineOD260/280≥2.0.
OD260/230≥2.0.
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity NumberPurity
Meta-transcriptome LibraryTotal RNA≥ 300 ng≥ 5.8, with flat baselineOD260/280≥2.0.
OD260/230≥2.0.
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity NumberPurity
Meta-transcriptome LibraryTotal RNA≥ 300 ng≥ 5.8, with flat baselineOD260/280≥2.0.
OD260/230≥2.0.
no degradation,
no contamination

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina Sequencing System
Read LengthPaired-end 150bp
Recommended Data Amount≥ 12 Gb per sample
Content of Data AnalysisData Quality Control
Metatranscriptome Assembly
Species Annotation
Functional Annotation
Taxonomic Analysis
Gene expression quantification & Differential expression analysis
Enrichment Analysis

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina Sequencing System
Read LengthPaired-end 150bp
Recommended Data Amount≥ 12 Gb per sample
Content of Data AnalysisData Quality Control
Metatranscriptome Assembly
Species Annotation
Functional Annotation
Taxonomic Analysis
Gene expression quantification & Differential expression analysis
Enrichment Analysis

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina Sequencing System
Read LengthPaired-end 150bp
Recommended Data Amount≥ 12 Gb per sample
Content of Data AnalysisData Quality Control
Metatranscriptome Assembly
Species Annotation
Functional Annotation
Taxonomic Analysis
Gene expression quantification & Differential expression analysis
Enrichment Analysis

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina Sequencing System
Read LengthPaired-end 150bp
Recommended Data Amount≥ 12 Gb per sample
Content of Data AnalysisData Quality Control
Metatranscriptome Assembly
Species Annotation
Functional Annotation
Taxonomic Analysis
Gene expression quantification & Differential expression analysis
Enrichment Analysis

Resources

Example Analysis Output

Image
Image
1/1
Pathway Enrichment Analysis

Identify enriched pathways associated with differentially expressed microbial genes for functional interpretation.

Image
Image
1/1
Active Species Heatmap

Visualise relative activity patterns of annotated microbial species across samples or groups.

Image
Image
1/1
Expression Volcano Plot

Highlight significantly changed microbial genes based on fold change and statistical significance.

Image
Image
1/1
Functional Categories

Summarise expressed genes by functional category to review community-level activity profiles.

Image
Image
1/1
KEGG Pathway Summary

Review KEGG pathway annotation results to support pathway-level interpretation of expressed genes

Image
Image
1/1
Sample Expression Clustering

Compare sample-level expression patterns to assess grouping, similarity and transcriptome variation.

Image
Image
1/1
Expression Overlap

Show shared and unique expressed genes across sample groups or comparison sets.

Image
Image
1/1
AMR Gene Overview

Visualise antimicrobial resistance-related gene patterns across samples or annotated categories.

Image
Image
1/1
Pathway Enrichment Analysis

Identify enriched pathways associated with differentially expressed microbial genes for functional interpretation.

Image
Image
1/1
Active Species Heatmap

Visualise relative activity patterns of annotated microbial species across samples or groups.

Image
Image
1/1
Expression Volcano Plot

Highlight significantly changed microbial genes based on fold change and statistical significance.

Image
Image
1/1
Functional Categories

Summarise expressed genes by functional category to review community-level activity profiles.

Image
Image
1/1
KEGG Pathway Summary

Review KEGG pathway annotation results to support pathway-level interpretation of expressed genes

Image
Image
1/1
Sample Expression Clustering

Compare sample-level expression patterns to assess grouping, similarity and transcriptome variation.

Image
Image
1/1
Expression Overlap

Show shared and unique expressed genes across sample groups or comparison sets.

Image
Image
1/1
AMR Gene Overview

Visualise antimicrobial resistance-related gene patterns across samples or annotated categories.

Image
Image
1/1
Pathway Enrichment Analysis

Identify enriched pathways associated with differentially expressed microbial genes for functional interpretation.

Image
Image
1/1
Active Species Heatmap

Visualise relative activity patterns of annotated microbial species across samples or groups.

Image
Image
1/1
Expression Volcano Plot

Highlight significantly changed microbial genes based on fold change and statistical significance.

Image
Image
1/1
Functional Categories

Summarise expressed genes by functional category to review community-level activity profiles.

Image
Image
1/1
KEGG Pathway Summary

Review KEGG pathway annotation results to support pathway-level interpretation of expressed genes

Image
Image
1/1
Sample Expression Clustering

Compare sample-level expression patterns to assess grouping, similarity and transcriptome variation.

Image
Image
1/1
Expression Overlap

Show shared and unique expressed genes across sample groups or comparison sets.

Image
Image
1/1
AMR Gene Overview

Visualise antimicrobial resistance-related gene patterns across samples or annotated categories.

Image
Image
1/1
Pathway Enrichment Analysis

Identify enriched pathways associated with differentially expressed microbial genes for functional interpretation.

Image
Image
1/1
Active Species Heatmap

Visualise relative activity patterns of annotated microbial species across samples or groups.

Image
Image
1/1
Expression Volcano Plot

Highlight significantly changed microbial genes based on fold change and statistical significance.

Image
Image
1/1
Functional Categories

Summarise expressed genes by functional category to review community-level activity profiles.

Image
Image
1/1
KEGG Pathway Summary

Review KEGG pathway annotation results to support pathway-level interpretation of expressed genes

Image
Image
1/1
Sample Expression Clustering

Compare sample-level expression patterns to assess grouping, similarity and transcriptome variation.

Image
Image
1/1
Expression Overlap

Show shared and unique expressed genes across sample groups or comparison sets.

Image
Image
1/1
AMR Gene Overview

Visualise antimicrobial resistance-related gene patterns across samples or annotated categories.

Webinars

Decoding Microbial Communities: Sequencing Solutions for Microbiome Research

Explore the latest approaches for studying microbial communities using amplicon sequencing, metagenomics, and metatranscriptomics. This webinar will highlight how these technologies can reveal microbial diversity, function, and interactions across human, environmental, and industrial ecosystems.

Learn More
(Decoding Microbial Communities: Sequencing Solutions for Microbiome Research)
Decoding Microbial Communities: Sequencing Solutions for Microbiome Research

Explore the latest approaches for studying microbial communities using amplicon sequencing, metagenomics, and metatranscriptomics. This webinar will highlight how these technologies can reveal microbial diversity, function, and interactions across human, environmental, and industrial ecosystems.

Learn More
(Decoding Microbial Communities: Sequencing Solutions for Microbiome Research)
Decoding Microbial Communities: Sequencing Solutions for Microbiome Research

Explore the latest approaches for studying microbial communities using amplicon sequencing, metagenomics, and metatranscriptomics. This webinar will highlight how these technologies can reveal microbial diversity, function, and interactions across human, environmental, and industrial ecosystems.

Learn More
(Decoding Microbial Communities: Sequencing Solutions for Microbiome Research)
Decoding Microbial Communities: Sequencing Solutions for Microbiome Research

Explore the latest approaches for studying microbial communities using amplicon sequencing, metagenomics, and metatranscriptomics. This webinar will highlight how these technologies can reveal microbial diversity, function, and interactions across human, environmental, and industrial ecosystems.

Learn More
(Decoding Microbial Communities: Sequencing Solutions for Microbiome Research)

Explore Transcriptomics Insights

Access webinars, brochures and case studies covering sequencing technologies, study planning and data interpretation.
View Webinar Library
(Explore Transcriptomics Insights)
View Webinar Library
(Explore Transcriptomics Insights)
Background
Background

Speak with a Specialist

Have a project in mind?

 

Send us your enquiry and a Novogene Europe specialist will be in touch.

 
 
 
 
 
 
 
 
 
 
Privacy PolicyCookie PolicyTerms
banner laptop bg
banner mobile bg

Metatranscriptome Sequencing

Community-wide RNA sequencing to explore active microbial gene expression, functional activity and environmental response.
OverviewOverview
BenefitsBenefits
ApplicationsApplications
SpecificationsSpecifications
ResourcesResources

Metatranscriptome sequencing enables transcriptome-wide analysis of actively expressed genes within complex microbial communities. Unlike DNA-based metagenomics, which profiles genetic potential, metatranscriptomics helps reveal which microbial genes and pathways are active under specific environmental, host-associated or experimental conditions.


Novogene Europe supports metatranscriptome sequencing projects using high-throughput RNA sequencing and bioinformatics workflows for metatranscriptome assembly, species annotation, functional annotation, taxonomic analysis, gene expression quantification and differential expression analysis. This approach can help researchers investigate microbial community function, pathway activity and adaptive responses across complex ecosystems.

Service Highlights

Active Community Expression Profiling
Active Community Expression Profiling

Profile expressed microbial genes across complex communities to investigate functional activity under specific host, environmental or experimental conditions.

Active Community Expression Profiling
Active Community Expression Profiling

Profile expressed microbial genes across complex communities to investigate functional activity under specific host, environmental or experimental conditions.

Taxonomic and Functional Annotation
Taxonomic and Functional Annotation

Bioinformatics workflows support species annotation, functional annotation and pathway-level interpretation of expressed microbial transcripts.

Taxonomic and Functional Annotation
Taxonomic and Functional Annotation

Bioinformatics workflows support species annotation, functional annotation and pathway-level interpretation of expressed microbial transcripts.

Metatranscriptome Assembly Support
Metatranscriptome Assembly Support

Assembly workflows help reconstruct expressed gene sequences from complex microbial RNA data, supporting downstream annotation and expression analysis.

Metatranscriptome Assembly Support
Metatranscriptome Assembly Support

Assembly workflows help reconstruct expressed gene sequences from complex microbial RNA data, supporting downstream annotation and expression analysis.

Differential Expression Analysis
Differential Expression Analysis

Compare microbial gene expression across sample groups to explore community-level functional changes, pathway activity and adaptive responses.

Differential Expression Analysis
Differential Expression Analysis

Compare microbial gene expression across sample groups to explore community-level functional changes, pathway activity and adaptive responses.

Service Highlights

Active Community Expression Profiling
Active Community Expression Profiling

Profile expressed microbial genes across complex communities to investigate functional activity under specific host, environmental or experimental conditions.

Active Community Expression Profiling
Active Community Expression Profiling

Profile expressed microbial genes across complex communities to investigate functional activity under specific host, environmental or experimental conditions.

Taxonomic and Functional Annotation
Taxonomic and Functional Annotation

Bioinformatics workflows support species annotation, functional annotation and pathway-level interpretation of expressed microbial transcripts.

Taxonomic and Functional Annotation
Taxonomic and Functional Annotation

Bioinformatics workflows support species annotation, functional annotation and pathway-level interpretation of expressed microbial transcripts.

Metatranscriptome Assembly Support
Metatranscriptome Assembly Support

Assembly workflows help reconstruct expressed gene sequences from complex microbial RNA data, supporting downstream annotation and expression analysis.

Metatranscriptome Assembly Support
Metatranscriptome Assembly Support

Assembly workflows help reconstruct expressed gene sequences from complex microbial RNA data, supporting downstream annotation and expression analysis.

Differential Expression Analysis
Differential Expression Analysis

Compare microbial gene expression across sample groups to explore community-level functional changes, pathway activity and adaptive responses.

Differential Expression Analysis
Differential Expression Analysis

Compare microbial gene expression across sample groups to explore community-level functional changes, pathway activity and adaptive responses.

Applications

Microbial Community Function

Metatranscriptome sequencing helps researchers investigate which microbial genes and pathways are actively expressed across complex community samples.

Microbial Community Function

Metatranscriptome sequencing helps researchers investigate which microbial genes and pathways are actively expressed across complex community samples.

Host–Microbe Interaction

Community RNA profiles can support studies of microbial activity in host-associated environments, including gut, skin, oral, infection or disease-related models.

Host–Microbe Interaction

Community RNA profiles can support studies of microbial activity in host-associated environments, including gut, skin, oral, infection or disease-related models.

Environmental and Ecological Response

Metatranscriptomics can help explore how microbial communities respond to environmental change, nutrient availability, stress or experimental treatments.

Environmental and Ecological Response

Metatranscriptomics can help explore how microbial communities respond to environmental change, nutrient availability, stress or experimental treatments.

Pathway and Functional Adaptation

Expressed gene and pathway analysis can support research into microbial metabolism, functional adaptation and community-level biological responses.

Pathway and Functional Adaptation

Expressed gene and pathway analysis can support research into microbial metabolism, functional adaptation and community-level biological responses.

Applications

Microbial Community Function

Metatranscriptome sequencing helps researchers investigate which microbial genes and pathways are actively expressed across complex community samples.

Microbial Community Function

Metatranscriptome sequencing helps researchers investigate which microbial genes and pathways are actively expressed across complex community samples.

Host–Microbe Interaction

Community RNA profiles can support studies of microbial activity in host-associated environments, including gut, skin, oral, infection or disease-related models.

Host–Microbe Interaction

Community RNA profiles can support studies of microbial activity in host-associated environments, including gut, skin, oral, infection or disease-related models.

Environmental and Ecological Response

Metatranscriptomics can help explore how microbial communities respond to environmental change, nutrient availability, stress or experimental treatments.

Environmental and Ecological Response

Metatranscriptomics can help explore how microbial communities respond to environmental change, nutrient availability, stress or experimental treatments.

Pathway and Functional Adaptation

Expressed gene and pathway analysis can support research into microbial metabolism, functional adaptation and community-level biological responses.

Pathway and Functional Adaptation

Expressed gene and pathway analysis can support research into microbial metabolism, functional adaptation and community-level biological responses.

Specifications

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity NumberPurity
Meta-transcriptome LibraryTotal RNA≥ 300 ng≥ 5.8, with flat baselineOD260/280≥2.0.
OD260/230≥2.0.
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity NumberPurity
Meta-transcriptome LibraryTotal RNA≥ 300 ng≥ 5.8, with flat baselineOD260/280≥2.0.
OD260/230≥2.0.
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity NumberPurity
Meta-transcriptome LibraryTotal RNA≥ 300 ng≥ 5.8, with flat baselineOD260/280≥2.0.
OD260/230≥2.0.
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity NumberPurity
Meta-transcriptome LibraryTotal RNA≥ 300 ng≥ 5.8, with flat baselineOD260/280≥2.0.
OD260/230≥2.0.
no degradation,
no contamination

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina Sequencing System
Read LengthPaired-end 150bp
Recommended Data Amount≥ 12 Gb per sample
Content of Data AnalysisData Quality Control
Metatranscriptome Assembly
Species Annotation
Functional Annotation
Taxonomic Analysis
Gene expression quantification & Differential expression analysis
Enrichment Analysis

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina Sequencing System
Read LengthPaired-end 150bp
Recommended Data Amount≥ 12 Gb per sample
Content of Data AnalysisData Quality Control
Metatranscriptome Assembly
Species Annotation
Functional Annotation
Taxonomic Analysis
Gene expression quantification & Differential expression analysis
Enrichment Analysis

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina Sequencing System
Read LengthPaired-end 150bp
Recommended Data Amount≥ 12 Gb per sample
Content of Data AnalysisData Quality Control
Metatranscriptome Assembly
Species Annotation
Functional Annotation
Taxonomic Analysis
Gene expression quantification & Differential expression analysis
Enrichment Analysis

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina Sequencing System
Read LengthPaired-end 150bp
Recommended Data Amount≥ 12 Gb per sample
Content of Data AnalysisData Quality Control
Metatranscriptome Assembly
Species Annotation
Functional Annotation
Taxonomic Analysis
Gene expression quantification & Differential expression analysis
Enrichment Analysis

Specifications

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity NumberPurity
Meta-transcriptome LibraryTotal RNA≥ 300 ng≥ 5.8, with flat baselineOD260/280≥2.0.
OD260/230≥2.0.
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity NumberPurity
Meta-transcriptome LibraryTotal RNA≥ 300 ng≥ 5.8, with flat baselineOD260/280≥2.0.
OD260/230≥2.0.
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity NumberPurity
Meta-transcriptome LibraryTotal RNA≥ 300 ng≥ 5.8, with flat baselineOD260/280≥2.0.
OD260/230≥2.0.
no degradation,
no contamination

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Library TypeSample TypeAmountRNA Integrity NumberPurity
Meta-transcriptome LibraryTotal RNA≥ 300 ng≥ 5.8, with flat baselineOD260/280≥2.0.
OD260/230≥2.0.
no degradation,
no contamination

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina Sequencing System
Read LengthPaired-end 150bp
Recommended Data Amount≥ 12 Gb per sample
Content of Data AnalysisData Quality Control
Metatranscriptome Assembly
Species Annotation
Functional Annotation
Taxonomic Analysis
Gene expression quantification & Differential expression analysis
Enrichment Analysis

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina Sequencing System
Read LengthPaired-end 150bp
Recommended Data Amount≥ 12 Gb per sample
Content of Data AnalysisData Quality Control
Metatranscriptome Assembly
Species Annotation
Functional Annotation
Taxonomic Analysis
Gene expression quantification & Differential expression analysis
Enrichment Analysis

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina Sequencing System
Read LengthPaired-end 150bp
Recommended Data Amount≥ 12 Gb per sample
Content of Data AnalysisData Quality Control
Metatranscriptome Assembly
Species Annotation
Functional Annotation
Taxonomic Analysis
Gene expression quantification & Differential expression analysis
Enrichment Analysis

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Sequencing PlatformIllumina Sequencing System
Read LengthPaired-end 150bp
Recommended Data Amount≥ 12 Gb per sample
Content of Data AnalysisData Quality Control
Metatranscriptome Assembly
Species Annotation
Functional Annotation
Taxonomic Analysis
Gene expression quantification & Differential expression analysis
Enrichment Analysis

Resources

Example Analysis Output

Image
Image
1/1
Pathway Enrichment Analysis

Identify enriched pathways associated with differentially expressed microbial genes for functional interpretation.

Image
Image
1/1
Active Species Heatmap

Visualise relative activity patterns of annotated microbial species across samples or groups.

Image
Image
1/1
Expression Volcano Plot

Highlight significantly changed microbial genes based on fold change and statistical significance.

Image
Image
1/1
Functional Categories

Summarise expressed genes by functional category to review community-level activity profiles.

Image
Image
1/1
KEGG Pathway Summary

Review KEGG pathway annotation results to support pathway-level interpretation of expressed genes

Image
Image
1/1
Sample Expression Clustering

Compare sample-level expression patterns to assess grouping, similarity and transcriptome variation.

Image
Image
1/1
Expression Overlap

Show shared and unique expressed genes across sample groups or comparison sets.

Image
Image
1/1
AMR Gene Overview

Visualise antimicrobial resistance-related gene patterns across samples or annotated categories.

Image
Image
1/1
Pathway Enrichment Analysis

Identify enriched pathways associated with differentially expressed microbial genes for functional interpretation.

Image
Image
1/1
Active Species Heatmap

Visualise relative activity patterns of annotated microbial species across samples or groups.

Image
Image
1/1
Expression Volcano Plot

Highlight significantly changed microbial genes based on fold change and statistical significance.

Image
Image
1/1
Functional Categories

Summarise expressed genes by functional category to review community-level activity profiles.

Image
Image
1/1
KEGG Pathway Summary

Review KEGG pathway annotation results to support pathway-level interpretation of expressed genes

Image
Image
1/1
Sample Expression Clustering

Compare sample-level expression patterns to assess grouping, similarity and transcriptome variation.

Image
Image
1/1
Expression Overlap

Show shared and unique expressed genes across sample groups or comparison sets.

Image
Image
1/1
AMR Gene Overview

Visualise antimicrobial resistance-related gene patterns across samples or annotated categories.

Image
Image
1/1
Pathway Enrichment Analysis

Identify enriched pathways associated with differentially expressed microbial genes for functional interpretation.

Image
Image
1/1
Active Species Heatmap

Visualise relative activity patterns of annotated microbial species across samples or groups.

Image
Image
1/1
Expression Volcano Plot

Highlight significantly changed microbial genes based on fold change and statistical significance.

Image
Image
1/1
Functional Categories

Summarise expressed genes by functional category to review community-level activity profiles.

Image
Image
1/1
KEGG Pathway Summary

Review KEGG pathway annotation results to support pathway-level interpretation of expressed genes

Image
Image
1/1
Sample Expression Clustering

Compare sample-level expression patterns to assess grouping, similarity and transcriptome variation.

Image
Image
1/1
Expression Overlap

Show shared and unique expressed genes across sample groups or comparison sets.

Image
Image
1/1
AMR Gene Overview

Visualise antimicrobial resistance-related gene patterns across samples or annotated categories.

Image
Image
1/1
Pathway Enrichment Analysis

Identify enriched pathways associated with differentially expressed microbial genes for functional interpretation.

Image
Image
1/1
Active Species Heatmap

Visualise relative activity patterns of annotated microbial species across samples or groups.

Image
Image
1/1
Expression Volcano Plot

Highlight significantly changed microbial genes based on fold change and statistical significance.

Image
Image
1/1
Functional Categories

Summarise expressed genes by functional category to review community-level activity profiles.

Image
Image
1/1
KEGG Pathway Summary

Review KEGG pathway annotation results to support pathway-level interpretation of expressed genes

Image
Image
1/1
Sample Expression Clustering

Compare sample-level expression patterns to assess grouping, similarity and transcriptome variation.

Image
Image
1/1
Expression Overlap

Show shared and unique expressed genes across sample groups or comparison sets.

Image
Image
1/1
AMR Gene Overview

Visualise antimicrobial resistance-related gene patterns across samples or annotated categories.

Webinars

Decoding Microbial Communities: Sequencing Solutions for Microbiome Research

Explore the latest approaches for studying microbial communities using amplicon sequencing, metagenomics, and metatranscriptomics. This webinar will highlight how these technologies can reveal microbial diversity, function, and interactions across human, environmental, and industrial ecosystems.

Learn More
(Decoding Microbial Communities: Sequencing Solutions for Microbiome Research)
Decoding Microbial Communities: Sequencing Solutions for Microbiome Research

Explore the latest approaches for studying microbial communities using amplicon sequencing, metagenomics, and metatranscriptomics. This webinar will highlight how these technologies can reveal microbial diversity, function, and interactions across human, environmental, and industrial ecosystems.

Learn More
(Decoding Microbial Communities: Sequencing Solutions for Microbiome Research)
Decoding Microbial Communities: Sequencing Solutions for Microbiome Research

Explore the latest approaches for studying microbial communities using amplicon sequencing, metagenomics, and metatranscriptomics. This webinar will highlight how these technologies can reveal microbial diversity, function, and interactions across human, environmental, and industrial ecosystems.

Learn More
(Decoding Microbial Communities: Sequencing Solutions for Microbiome Research)
Decoding Microbial Communities: Sequencing Solutions for Microbiome Research

Explore the latest approaches for studying microbial communities using amplicon sequencing, metagenomics, and metatranscriptomics. This webinar will highlight how these technologies can reveal microbial diversity, function, and interactions across human, environmental, and industrial ecosystems.

Learn More
(Decoding Microbial Communities: Sequencing Solutions for Microbiome Research)

Explore Transcriptomics Insights

Access webinars, brochures and case studies covering sequencing technologies, study planning and data interpretation.
View Webinar Library
(Explore Transcriptomics Insights)
View Webinar Library
(Explore Transcriptomics Insights)
Background
Background

Speak with a Specialist

Have a project in mind?

 

Send us your enquiry and a Novogene Europe specialist will be in touch.

 
 
 
 
 
 
 
 
 
 
Privacy PolicyCookie PolicyTerms