Sequencing Only on Illumina Sequencer

Sequencing Only on Illumina® Sequencer

High-quality short-read sequencing of customer-prepared libraries on the Illumina platform to support diverse genomic and transcriptomic applications.

Novogene Europe provides sequencing-only services for customer-prepared, ready-to-sequence libraries on Illumina platforms. This service is suitable for researchers who have completed library preparation in-house or through a third party and require reliable short-read sequencing with flexible data output options.

With support for different read lengths, project scales and library types, sequencing-only service can be used for a wide range of NGS applications, including genomic, transcriptomic, targeted and single-cell sequencing libraries. Standardised workflows and quality control processes support consistent sequencing execution, with project data delivered according to the agreed output requirements.

Service Highlights

Ready-to-sequence Library Support

Sequencing-only service for customer-prepared libraries that have completed library preparation and are ready for Illumina sequencing, supporting a broad range of NGS library types and applications.

Flexible Run Configuration

Support for different read lengths, sequencing output levels and project scales, helping researchers align sequencing setup with library design, data requirements, application needs and downstream analysis plans.

Regional Sequencing Capacity

Access to Novogene Europe’s regional high-throughput Illumina sequencing infrastructure, supporting efficient project scheduling and fast, reliable sequencing delivery for European researchers.

QC and Data Delivery

Library and sequencing quality checks support consistent project execution, with data delivered according to the agreed output requirements.

Applications

Prepared Libraries from Your Lab

Sequencing support for prepared DNA libraries used in whole genome, targeted, variant analysis or other genomic research workflows.

Third-party Prepared Libraries

For projects where libraries have already been prepared by another provider, collaborator or core facility and require sequencing-only support.

Flexible Sequencing Projects

For studies requiring specific read lengths, output levels, index configurations or pooling strategies, including prepared WGS, RNA-seq, targeted panel or single-cell libraries.

Scalable Project Delivery

For projects ranging from small pilot runs to larger sequencing batches, supported by Novogene Europe’s regional sequencing infrastructure.

Specifications

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Platform	Sequencing Platform	Data amount	Volume Requirement	Library Concentration
NovaSeq X Plus PE150	NovaSeq X Plus Partial Lane Seq	<30 Gb	≥15 μL	≥ 2 ng/μL, quantified by Qubit® 2.0 (Life Technologies) ≥2 nM, quantified by qPCR
		30 Gb ≤ X < 100 Gb	≥ 30 μL
		100 Gb ≤ X < 375 Gb	≥70 μL
		375 Gb ≤ X < 1 Tb	≥130 μL
	NovaSeq X Plus 10B	Lane sequencing on 10B	≥70 μL (additional 70 μL for one more lane)
	NovaSeq X Plus 25B	Lane sequencing on 25B	≥130 μL (additional 130 μL for one more lane)
NovaSeq series SE50/PE50/PE250	NovaSeq Partial Lane Seq	<20 M reads	≥15 μL
		20 M reads ≤ X < 50 M reads	≥25 μL
		50 M reads ≤ X ≤ 150 M reads	≥50 μL
	NovaSeq Lane Seq	Lane sequencing	≥70 μL (additional 70 μL for one more lane)

Notes: High concentration samples should be diluted before delivery. Pre-made libraries should be colourless. Click here to view the complete details of our sample requirements.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Platform	Sequencing Platform	Data amount	Volume Requirement	Library Concentration
NovaSeq X Plus PE150	NovaSeq X Plus Partial Lane Seq	<30 Gb	≥15 μL	≥ 2 ng/μL, quantified by Qubit® 2.0 (Life Technologies) ≥2 nM, quantified by qPCR
		30 Gb ≤ X < 100 Gb	≥ 30 μL
		100 Gb ≤ X < 375 Gb	≥70 μL
		375 Gb ≤ X < 1 Tb	≥130 μL
	NovaSeq X Plus 10B	Lane sequencing on 10B	≥70 μL (additional 70 μL for one more lane)
	NovaSeq X Plus 25B	Lane sequencing on 25B	≥130 μL (additional 130 μL for one more lane)
NovaSeq series SE50/PE50/PE250	NovaSeq Partial Lane Seq	<20 M reads	≥15 μL
		20 M reads ≤ X < 50 M reads	≥25 μL
		50 M reads ≤ X ≤ 150 M reads	≥50 μL
	NovaSeq Lane Seq	Lane sequencing	≥70 μL (additional 70 μL for one more lane)

Notes: High concentration samples should be diluted before delivery. Pre-made libraries should be colourless. Click here to view the complete details of our sample requirements.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Platform	Sequencing Platform	Data amount	Volume Requirement	Library Concentration
NovaSeq X Plus PE150	NovaSeq X Plus Partial Lane Seq	<30 Gb	≥15 μL	≥ 2 ng/μL, quantified by Qubit® 2.0 (Life Technologies) ≥2 nM, quantified by qPCR
		30 Gb ≤ X < 100 Gb	≥ 30 μL
		100 Gb ≤ X < 375 Gb	≥70 μL
		375 Gb ≤ X < 1 Tb	≥130 μL
	NovaSeq X Plus 10B	Lane sequencing on 10B	≥70 μL (additional 70 μL for one more lane)
	NovaSeq X Plus 25B	Lane sequencing on 25B	≥130 μL (additional 130 μL for one more lane)
NovaSeq series SE50/PE50/PE250	NovaSeq Partial Lane Seq	<20 M reads	≥15 μL
		20 M reads ≤ X < 50 M reads	≥25 μL
		50 M reads ≤ X ≤ 150 M reads	≥50 μL
	NovaSeq Lane Seq	Lane sequencing	≥70 μL (additional 70 μL for one more lane)

Notes: High concentration samples should be diluted before delivery. Pre-made libraries should be colourless. Click here to view the complete details of our sample requirements.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Platform	Sequencing Platform	Data amount	Volume Requirement	Library Concentration
NovaSeq X Plus PE150	NovaSeq X Plus Partial Lane Seq	<30 Gb	≥15 μL	≥ 2 ng/μL, quantified by Qubit® 2.0 (Life Technologies) ≥2 nM, quantified by qPCR
		30 Gb ≤ X < 100 Gb	≥ 30 μL
		100 Gb ≤ X < 375 Gb	≥70 μL
		375 Gb ≤ X < 1 Tb	≥130 μL
	NovaSeq X Plus 10B	Lane sequencing on 10B	≥70 μL (additional 70 μL for one more lane)
	NovaSeq X Plus 25B	Lane sequencing on 25B	≥130 μL (additional 130 μL for one more lane)
NovaSeq series SE50/PE50/PE250	NovaSeq Partial Lane Seq	<20 M reads	≥15 μL
		20 M reads ≤ X < 50 M reads	≥25 μL
		50 M reads ≤ X ≤ 150 M reads	≥50 μL
	NovaSeq Lane Seq	Lane sequencing	≥70 μL (additional 70 μL for one more lane)

Notes: High concentration samples should be diluted before delivery. Pre-made libraries should be colourless. Click here to view the complete details of our sample requirements.

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Platform	Read Length	Data Output
NovaSeq X Plus	PE150（10B ）	375Gb/lane 3 Tb/flow cell
	PE150（25B ）	1 Tb/lane 8 Tb/flow cell
	PE50 (1.5B)	1.5B flow cell 75 Gb/lane 150 Gb/flow cell
	SE50 (1.5B)	1.5B flow cell 38Gb/lane 75 Gb/flow cell
NovaSeq 6000	PE250	200 Gb/lane 400 Gb/flow cell

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Platform	Read Length	Data Output
NovaSeq X Plus	PE150（10B ）	375Gb/lane 3 Tb/flow cell
	PE150（25B ）	1 Tb/lane 8 Tb/flow cell
	PE50 (1.5B)	1.5B flow cell 75 Gb/lane 150 Gb/flow cell
	SE50 (1.5B)	1.5B flow cell 38Gb/lane 75 Gb/flow cell
NovaSeq 6000	PE250	200 Gb/lane 400 Gb/flow cell

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Platform	Read Length	Data Output
NovaSeq X Plus	PE150（10B ）	375Gb/lane 3 Tb/flow cell
	PE150（25B ）	1 Tb/lane 8 Tb/flow cell
	PE50 (1.5B)	1.5B flow cell 75 Gb/lane 150 Gb/flow cell
	SE50 (1.5B)	1.5B flow cell 38Gb/lane 75 Gb/flow cell
NovaSeq 6000	PE250	200 Gb/lane 400 Gb/flow cell

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Platform	Read Length	Data Output
NovaSeq X Plus	PE150（10B ）	375Gb/lane 3 Tb/flow cell
	PE150（25B ）	1 Tb/lane 8 Tb/flow cell
	PE50 (1.5B)	1.5B flow cell 75 Gb/lane 150 Gb/flow cell
	SE50 (1.5B)	1.5B flow cell 38Gb/lane 75 Gb/flow cell
NovaSeq 6000	PE250	200 Gb/lane 400 Gb/flow cell

Specifications

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Platform	Sequencing Platform	Data amount	Volume Requirement	Library Concentration
NovaSeq X Plus PE150	NovaSeq X Plus Partial Lane Seq	<30 Gb	≥15 μL	≥ 2 ng/μL, quantified by Qubit® 2.0 (Life Technologies) ≥2 nM, quantified by qPCR
		30 Gb ≤ X < 100 Gb	≥ 30 μL
		100 Gb ≤ X < 375 Gb	≥70 μL
		375 Gb ≤ X < 1 Tb	≥130 μL
	NovaSeq X Plus 10B	Lane sequencing on 10B	≥70 μL (additional 70 μL for one more lane)
	NovaSeq X Plus 25B	Lane sequencing on 25B	≥130 μL (additional 130 μL for one more lane)
NovaSeq series SE50/PE50/PE250	NovaSeq Partial Lane Seq	<20 M reads	≥15 μL
		20 M reads ≤ X < 50 M reads	≥25 μL
		50 M reads ≤ X ≤ 150 M reads	≥50 μL
	NovaSeq Lane Seq	Lane sequencing	≥70 μL (additional 70 μL for one more lane)

Notes: High concentration samples should be diluted before delivery. Pre-made libraries should be colourless. Click here to view the complete details of our sample requirements.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Platform	Sequencing Platform	Data amount	Volume Requirement	Library Concentration
NovaSeq X Plus PE150	NovaSeq X Plus Partial Lane Seq	<30 Gb	≥15 μL	≥ 2 ng/μL, quantified by Qubit® 2.0 (Life Technologies) ≥2 nM, quantified by qPCR
		30 Gb ≤ X < 100 Gb	≥ 30 μL
		100 Gb ≤ X < 375 Gb	≥70 μL
		375 Gb ≤ X < 1 Tb	≥130 μL
	NovaSeq X Plus 10B	Lane sequencing on 10B	≥70 μL (additional 70 μL for one more lane)
	NovaSeq X Plus 25B	Lane sequencing on 25B	≥130 μL (additional 130 μL for one more lane)
NovaSeq series SE50/PE50/PE250	NovaSeq Partial Lane Seq	<20 M reads	≥15 μL
		20 M reads ≤ X < 50 M reads	≥25 μL
		50 M reads ≤ X ≤ 150 M reads	≥50 μL
	NovaSeq Lane Seq	Lane sequencing	≥70 μL (additional 70 μL for one more lane)

Notes: High concentration samples should be diluted before delivery. Pre-made libraries should be colourless. Click here to view the complete details of our sample requirements.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Platform	Sequencing Platform	Data amount	Volume Requirement	Library Concentration
NovaSeq X Plus PE150	NovaSeq X Plus Partial Lane Seq	<30 Gb	≥15 μL	≥ 2 ng/μL, quantified by Qubit® 2.0 (Life Technologies) ≥2 nM, quantified by qPCR
		30 Gb ≤ X < 100 Gb	≥ 30 μL
		100 Gb ≤ X < 375 Gb	≥70 μL
		375 Gb ≤ X < 1 Tb	≥130 μL
	NovaSeq X Plus 10B	Lane sequencing on 10B	≥70 μL (additional 70 μL for one more lane)
	NovaSeq X Plus 25B	Lane sequencing on 25B	≥130 μL (additional 130 μL for one more lane)
NovaSeq series SE50/PE50/PE250	NovaSeq Partial Lane Seq	<20 M reads	≥15 μL
		20 M reads ≤ X < 50 M reads	≥25 μL
		50 M reads ≤ X ≤ 150 M reads	≥50 μL
	NovaSeq Lane Seq	Lane sequencing	≥70 μL (additional 70 μL for one more lane)

Notes: High concentration samples should be diluted before delivery. Pre-made libraries should be colourless. Click here to view the complete details of our sample requirements.

Sample Requirements

The quantities listed are provided for reference only. Please download the Sample Requirements for detailed guidance, or contact us to discuss your project-specific requirements.

Platform	Sequencing Platform	Data amount	Volume Requirement	Library Concentration
NovaSeq X Plus PE150	NovaSeq X Plus Partial Lane Seq	<30 Gb	≥15 μL	≥ 2 ng/μL, quantified by Qubit® 2.0 (Life Technologies) ≥2 nM, quantified by qPCR
		30 Gb ≤ X < 100 Gb	≥ 30 μL
		100 Gb ≤ X < 375 Gb	≥70 μL
		375 Gb ≤ X < 1 Tb	≥130 μL
	NovaSeq X Plus 10B	Lane sequencing on 10B	≥70 μL (additional 70 μL for one more lane)
	NovaSeq X Plus 25B	Lane sequencing on 25B	≥130 μL (additional 130 μL for one more lane)
NovaSeq series SE50/PE50/PE250	NovaSeq Partial Lane Seq	<20 M reads	≥15 μL
		20 M reads ≤ X < 50 M reads	≥25 μL
		50 M reads ≤ X ≤ 150 M reads	≥50 μL
	NovaSeq Lane Seq	Lane sequencing	≥70 μL (additional 70 μL for one more lane)

Notes: High concentration samples should be diluted before delivery. Pre-made libraries should be colourless. Click here to view the complete details of our sample requirements.

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Platform	Read Length	Data Output
NovaSeq X Plus	PE150（10B ）	375Gb/lane 3 Tb/flow cell
	PE150（25B ）	1 Tb/lane 8 Tb/flow cell
	PE50 (1.5B)	1.5B flow cell 75 Gb/lane 150 Gb/flow cell
	SE50 (1.5B)	1.5B flow cell 38Gb/lane 75 Gb/flow cell
NovaSeq 6000	PE250	200 Gb/lane 400 Gb/flow cell

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Platform	Read Length	Data Output
NovaSeq X Plus	PE150（10B ）	375Gb/lane 3 Tb/flow cell
	PE150（25B ）	1 Tb/lane 8 Tb/flow cell
	PE50 (1.5B)	1.5B flow cell 75 Gb/lane 150 Gb/flow cell
	SE50 (1.5B)	1.5B flow cell 38Gb/lane 75 Gb/flow cell
NovaSeq 6000	PE250	200 Gb/lane 400 Gb/flow cell

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Platform	Read Length	Data Output
NovaSeq X Plus	PE150（10B ）	375Gb/lane 3 Tb/flow cell
	PE150（25B ）	1 Tb/lane 8 Tb/flow cell
	PE50 (1.5B)	1.5B flow cell 75 Gb/lane 150 Gb/flow cell
	SE50 (1.5B)	1.5B flow cell 38Gb/lane 75 Gb/flow cell
NovaSeq 6000	PE250	200 Gb/lane 400 Gb/flow cell

Sequencing and Analysis

Recommended data outputs and analysis examples are provided for reference only. For detailed information, please contact us to discuss your project-specific analysis requirements.

Platform	Read Length	Data Output
NovaSeq X Plus	PE150（10B ）	375Gb/lane 3 Tb/flow cell
	PE150（25B ）	1 Tb/lane 8 Tb/flow cell
	PE50 (1.5B)	1.5B flow cell 75 Gb/lane 150 Gb/flow cell
	SE50 (1.5B)	1.5B flow cell 38Gb/lane 75 Gb/flow cell
NovaSeq 6000	PE250	200 Gb/lane 400 Gb/flow cell

Resources

Example Analysis Output

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Quality Score Distribution

Review base quality scores across sequencing reads to assess overall read quality and sequencing performance.

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Error Rate Distribution

Visualise estimated error rates across read positions to support sequencing quality assessment and run-level review.

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Base Content Distribution

Assess base composition across read positions to review sequence balance and library-specific patterns.

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Raw Read Classification

Summarise clean reads, low-quality reads and adapter-related reads after raw data filtering.

Webinars

Preparing Premade Libraries for High-Quality Sequencing Across Multiple Platforms

Discover how to prepare premade libraries that perform optimally across Illumina, Element, and PacBio sequencing platforms. We will discuss adapter design, indexing requirements, quality control, and workflow best practices to help ensure reliable, high-quality sequencing results.

Learn More

Explore Library Sequencing Support

Access webinars, brochures and case studies covering sequencing technologies, study planning and data interpretation.

Speak with a Specialist

Have a project in mind?

Send us your enquiry and a Novogene Europe specialist will be in touch.