Novogene, a leading provider of genomic sequencing services, has recently installed the latest PacBio Revio long-read sequencing system in its US and Chinese laboratories. This cutting-edge technology offers throughput of 360 Gb of HiFi reads per day, a 15× increase compared to PacBio’s previous system, the Sequel IIe. Novogene has conducted validation runs with DNA HiFi libraries of plant, animal, and human samples. The results reveal an output of 110.5Gb per SMRT cell, which surpasses the standard data output of 90Gb/cell officially promoted by PacBio.. The enhanced capabilities of the Revio system will allow Novogene to provide high throughput for HiFi long read sequencing and direct methylation detection for a wide array of applications.

1. HiFi yield per Revio SMRT cell:

The average data output per SMRT cell is 98.4Gb, with a quality value of 31.2. Additionally, the platform demonstrated a maximum data output of 110.5 Gb per SMRT cell.

Table 1. Quality control results for Revio sequencing data 2. Testing results of multiplexed libraries

Multiplexed DNA libraries, consisting of three animal samples and three plant samples, were sequenced on the Revio platform respectively. The results showed that each animal sample yielded more than 30Gb HiFi reads, and the data demultiplexing rate reaches a level of more than 99%.

Table 2. Quality control results of multiplexed SMRT cell libraries 3. Comparison of sequencing data achieved on Sequel II and Revio

Human and plant gDNA samples were used for HiFi library preparation and sequenced on Pacbio’s Sequel II and Revio systems respectively, to evaluate the difference in performance. The results indicate that the Revio system was able to yield 2-3 times more HiFi data per SMRT cell compared to the Sequel II. Furthermore, Revio completed the sequencing in a shorter run time, while maintaining a mean data quality > Q30.

Table 3. Sequel II vs Revio sequencing data 4. Data analysis of human whole genome sequencing on Revio

The achieved mapping rate of a human whole genome was 99.91%, with coverage across the entire genome of 99.64% and an average sequencing depth of 38X.

Table 4. Mapping statistics of human samples sequenced on the Revio system. Figure 1 (a). Statistics of structural variation length; Figure 1(b). Density of variant chromosome distribution, including translocation, insertion, deletion, inversion, copy number variant, short tandem repeat, gene, and chromosome. About Novogene

Novogene accumulated extensive experience in third-generation library preparation, sequencing, and bioinformatics analysis across numerous species. Novogene has technical advantages in de novo sequencing technology and applications, holding 18 patent licenses and over 40 software copyrights, and has jointly published over 109 papers in international journals.

With one of the largest sequencing capacities in the world, we utilize our deep scientific knowledge, first-class customer service, and unsurpassed data quality to help clients realize their research goals in the rapidly evolving world of genomics. Novogene is committed to becoming your trusted genomics partner.